› 6s2f

Electron Microscopy
5.8Å resolution

Cryo-EM structure of Ctf18-1-8 in complex with the catalytic domain of DNA polymerase epsilon (Class 2)

Released:
DOI: 10.2210/pdb6s2f/pdb
PDB entry 6s2f coloured by chain, front view.
PDB entry 6s2f coloured by chain, side view.
PDB entry 6s2f coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Ctf18-RFC and DNA Pol ϵ form a stable leading strand polymerase/clamp loader complex required for normal and perturbed DNA replication.
Stokes K, Winczura A, Song B, Piccoli G, Grabarczyk DB
OpenAccess logo Nucleic Acids Res 48 8128-8145 (2020)
PMID: 32585006
Related structures: EMD-10089

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-149481 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
DNA polymerase epsilon catalytic subunit A Chain: A
Molecule details ›
Chain: A
Length: 1219 amino acids
Theoretical weight: 140.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P21951 (Residues: 1-1192; Coverage: 54%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains:
Chromosome transmission fidelity protein 8 Chain: D
Molecule details ›
Chain: D
Length: 133 amino acids
Theoretical weight: 15.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38877 (Residues: 1-133; Coverage: 100%)
Gene names: CTF8, YHR191C
Sequence domains: Ctf8
Chromosome transmission fidelity protein 18 Chain: E
Molecule details ›
Chain: E
Length: 33 amino acids
Theoretical weight: 3.86 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P49956 (Residues: 713-741; Coverage: 4%)
Gene names: CHL12, CTF18, YM9582.03C, YMR078C
Sister chromatid cohesion protein DCC1 Chain: B
Molecule details ›
Chain: B
Length: 380 amino acids
Theoretical weight: 44.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P25559 (Residues: 1-380; Coverage: 100%)
Gene names: DCC1, YCL016C, YCL16C
Sequence domains: Sister chromatid cohesion protein Dcc1

Ligands and Environments

1 bound ligand:
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.8Å
Relevant EMDB volumes: EMD-10089
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Eukaryotic clamp loaders and unloaders in the maintenance of genome stability.
Lee et al. (2020)
5 more

1 mention without citation

Ctf18-RFC and DNA Pol ϵ form a stable leading strand polymerase/clamp loader complex required for normal and perturbed DNA replication.
Stokes et al. (2020)