› 6s8k

X-ray diffraction
1.52Å resolution

Structure, Thermodynamics, and Kinetics of Plinabulin Binding to two Tubulin Isotypes

Released:
DOI: 10.2210/pdb6s8k/pdb
PDB entry 6s8k coloured by chain, front view.
PDB entry 6s8k coloured by chain, side view.
PDB entry 6s8k coloured by chain, top view.
Source organisms:
Primary publication:
Structure, Thermodynamics, and Kinetics of Plinabulin Binding to Two Tubulin Isotypes
La Sala G, Olieric N, Sharma A
Chem 5 (2019)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-160568 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 48.67 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-437; Coverage: 97%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Designed ankyrin repeat protein (DARPIN) D1 Chain: F
Molecule details ›
Chain: F
Length: 155 amino acids
Theoretical weight: 16.44 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand PN6 1 x PN6
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 73.565Å b: 91.351Å c: 83.221Å
α: 90° β: 96.851° γ: 90°
R-values:
R R work R free
0.179 0.177 0.203
Expression system: Escherichia coli

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