› 6s8l

X-ray diffraction
1.8Å resolution

Structure, Thermodynamics, and Kinetics of Plinabulin Binding to two Tubulin Isotypes

Released:
DOI: 10.2210/pdb6s8l/pdb
PDB entry 6s8l coloured by chain, front view.
PDB entry 6s8l coloured by chain, side view.
PDB entry 6s8l coloured by chain, top view.
Source organisms:
Primary publication:
Structure, Thermodynamics, and Kinetics of Plinabulin Binding to Two Tubulin Isotypes
La Sala G, Olieric N
Chem (2019)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-549889 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P68363 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta-3 chain Chain: B
Molecule details ›
Chain: B
Length: 450 amino acids
Theoretical weight: 50.48 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q13509 (Residues: 1-450; Coverage: 100%)
Gene names: TUBB3, TUBB4
Sequence domains:
Designed ankyrin repeat protein (DARPIN) D1 Chain: F
Molecule details ›
Chain: F
Length: 157 amino acids
Theoretical weight: 16.66 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 2 x MG
Ligand PN6 1 x PN6
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 73.63Å b: 91.2Å c: 82.64Å
α: 90° β: 97.49° γ: 90°
R-values:
R R work R free
0.218 0.215 0.266
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

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