› 6s8q

X-ray diffraction
2.39Å resolution

Human Brr2 Helicase Region in complex with C-tail deleted Jab1

Released:
DOI: 10.2210/pdb6s8q/pdb
PDB entry 6s8q coloured by chain, front view.
PDB entry 6s8q coloured by chain, side view.
PDB entry 6s8q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The inactive C-terminal cassette of the dual-cassette RNA helicase BRR2 both stimulates and inhibits the activity of the N-terminal helicase unit.
Vester K, Santos KF, Kuropka B, Weise C, Wahl MC
J Biol Chem 295 2097-2112 (2020)
PMID: 31914407

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131091 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
U5 small nuclear ribonucleoprotein 200 kDa helicase Chain: B
Molecule details ›
Chain: B
Length: 1747 amino acids
Theoretical weight: 199.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75643 (Residues: 394-2136; Coverage: 82%)
Gene names: ASCC3L1, BRR2, HELIC2, KIAA0788, SNRNP200
Sequence domains:
Pre-mRNA-processing-splicing factor 8 Chain: J
Molecule details ›
Chain: J
Length: 263 amino acids
Theoretical weight: 30.13 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6P2Q9 (Residues: 2064-2320; Coverage: 11%)
Gene names: PRPC8, PRPF8
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 99.48Å b: 118.632Å c: 187.716Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.253
Expression system: Trichoplusia ni

Quick links

Citations

1 review citation

Probing the structure and function of polymerase θ helicase-like domain.
Vanson et al. (2022)
4 other articles

4 mentions without citation

The inactive C-terminal cassette of the dual-cassette RNA helicase BRR2 both stimulates and inhibits the activity of the N-terminal helicase unit.
Vester et al. (2020)
3 more