› 6sb2

Electron Microscopy
6.2Å resolution

cryo-EM structure of mTORC1 bound to active RagA/C GTPases

Released:
DOI: 10.2210/pdb6sb2/pdb
PDB entry 6sb2 coloured by chain, front view.
PDB entry 6sb2 coloured by chain, side view.
PDB entry 6sb2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Architecture of human Rag GTPase heterodimers and their complex with mTORC1.
Anandapadamanaban M, Masson GR, Perisic O, Berndt A, Kaufman J, Johnson CM, Santhanam B, Rogala KB, Sabatini DM, Williams RL
Science 366 203-210 (2019)
PMID: 31601764
Related structures: EMD-10133

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154635 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Serine/threonine-protein kinase mTOR Chains: A, B
Molecule details ›
Chains: A, B
Length: 2549 amino acids
Theoretical weight: 287.24 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P42345 (Residues: 54-2549; Coverage: 98%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Target of rapamycin complex subunit LST8 Chains: E, H
Molecule details ›
Chains: E, H
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat
Ras-related GTP-binding protein A Chains: C, I
Molecule details ›
Chains: C, I
Length: 313 amino acids
Theoretical weight: 36.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7L523 (Residues: 1-313; Coverage: 100%)
Gene name: RRAGA
Sequence domains: Gtr1/RagA G protein conserved region
Ras-related GTP-binding protein C Chains: D, J
Molecule details ›
Chains: D, J
Length: 399 amino acids
Theoretical weight: 44.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HB90 (Residues: 1-399; Coverage: 100%)
Gene name: RRAGC
Sequence domains: Gtr1/RagA G protein conserved region
Regulatory-associated protein of mTOR Chains: N, Y
Molecule details ›
Chains: N, Y
Length: 1335 amino acids
Theoretical weight: 149.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N122 (Residues: 1-1335; Coverage: 100%)
Gene names: KIAA1303, RAPTOR, RPTOR
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.2Å
Relevant EMDB volumes: EMD-10133
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

16 review citations

The mTOR-Autophagy Axis and the Control of Metabolism.
Deleyto-Seldas et al. (2021)
15 more

1 mention without citation

Architecture of human Rag GTPase heterodimers and their complex with mTORC1.
Anandapadamanaban et al. (2019)