6scp › Citations

Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum.

<div class='caption-title'>Phenotypic characterization of Pino-sepF.</div>
<div class='caption-title'>Molecular characterization of the SepF–FtsZ interaction.</div>
<div class='caption-title'>Complementation and localization of SepF mutants in the Pino-sepF strain.</div>
Sogues A, Martinez M, Gaday Q, Ben Assaya M, Graña M, Voegele A, VanNieuwenhze M, England P, Haouz A, Chenal A, Trépout S, Duran R, Wehenkel AM, Alzari PM
OpenAccess logo Nat Commun 11 1641 (2020)
Related entries: 6sat, 6scq, 6scs

Cited: 16 times
EuropePMC logo PMID: 32242019

Abstract

The mechanisms of Z-ring assembly and regulation in bacteria are poorly understood, particularly in non-model organisms. Actinobacteria, a large bacterial phylum that includes the pathogen Mycobacterium tuberculosis, lack the canonical FtsZ-membrane anchors and Z-ring regulators described for E. coli. Here we investigate the physiological function of Corynebacterium glutamicum SepF, the only cell division-associated protein from Actinobacteria known to interact with the conserved C-terminal tail of FtsZ. We show an essential interdependence of FtsZ and SepF for formation of a functional Z-ring in C. glutamicum. The crystal structure of the SepF-FtsZ complex reveals a hydrophobic FtsZ-binding pocket, which defines the SepF homodimer as the functional unit, and suggests a reversible oligomerization interface. FtsZ filaments and lipid membranes have opposing effects on SepF polymerization, indicating that SepF has multiple roles at the cell division site, involving FtsZ bundling, Z-ring tethering and membrane reshaping activities that are needed for proper Z-ring assembly and function.

Articles - 6scp mentioned but not cited (2)

  1. Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum. Sogues A, Martinez M, Gaday Q, Ben Assaya M, Graña M, Voegele A, VanNieuwenhze M, England P, Haouz A, Chenal A, Trépout S, Duran R, Wehenkel AM, Alzari PM. Nat Commun 11 1641 (2020)
  2. SepF is the FtsZ anchor in archaea, with features of an ancestral cell division system. Pende N, Sogues A, Megrian D, Sartori-Rupp A, England P, Palabikyan H, Rittmann SKR, Graña M, Wehenkel AM, Alzari PM, Gribaldo S. Nat Commun 12 3214 (2021)


Reviews citing this publication (4)

  1. Simple, efficient and thorough shotgun proteomic analysis with PatternLab V. Santos MDM, Lima DB, Fischer JSG, Clasen MA, Kurt LU, Camillo-Andrade AC, Monteiro LC, de Aquino PF, Neves-Ferreira AGC, Valente RH, Trugilho MRO, Brunoro GVF, Souza TACB, Santos RM, Batista M, Gozzo FC, Durán R, Yates JR, Barbosa VC, Carvalho PC. Nat Protoc 17 1553-1578 (2022)
  2. ylm Has More than a (Z Anchor) Ring to It! White ML, Eswara PJ. J Bacteriol 203 e00460-20 (2021)
  3. Models versus pathogens: how conserved is the FtsZ in bacteria? Battaje RR, Piyush R, Pratap V, Panda D. Biosci Rep 43 BSR20221664 (2023)
  4. Anchors: A way for FtsZ filaments to stay membrane bound. Naha A, Haeusser DP, Margolin W. Mol Microbiol 120 525-538 (2023)

Articles citing this publication (10)

  1. Population genomics and antimicrobial resistance in Corynebacterium diphtheriae. Hennart M, Panunzi LG, Rodrigues C, Gaday Q, Baines SL, Barros-Pinkelnig M, Carmi-Leroy A, Dazas M, Wehenkel AM, Didelot X, Toubiana J, Badell E, Brisse S. Genome Med 12 107 (2020)
  2. Single-molecule imaging reveals that Z-ring condensation is essential for cell division in Bacillus subtilis. Squyres GR, Holmes MJ, Barger SR, Pennycook BR, Ryan J, Yan VT, Garner EC. Nat Microbiol 6 553-562 (2021)
  3. Dissecting the Functional Contributions of the Intrinsically Disordered C-terminal Tail of Bacillus subtilis FtsZ. Cohan MC, Eddelbuettel AMP, Levin PA, Pappu RV. J Mol Biol 432 3205-3221 (2020)
  4. The archaeal protein SepF is essential for cell division in Haloferax volcanii. Nußbaum P, Gerstner M, Dingethal M, Erb C, Albers SV. Nat Commun 12 3469 (2021)
  5. Enabling reactive microscopy with MicroMator. Fox ZR, Fletcher S, Fraisse A, Aditya C, Sosa-Carrillo S, Petit J, Gilles S, Bertaux F, Ruess J, Batt G. Nat Commun 13 2199 (2022)
  6. Membrane Tethering of SepF, a Membrane Anchor for the Mycobacterium tuberculosis Z-ring. Dey S, Zhou HX. J Mol Biol 434 167817 (2022)
  7. ReSMAP: Web Server for Predicting Residue-Specific Membrane-Association Propensities of Intrinsically Disordered Proteins. Qin S, Hicks A, Dey S, Prasad R, Zhou HX. Membranes (Basel) 12 773 (2022)
  8. An Arg/Ala-rich helix in the N-terminal region of M. tuberculosis FtsQ is a potential membrane anchor of the Z-ring. Smrt ST, Escobar CA, Dey S, Cross TA, Zhou HX. Commun Biol 6 311 (2023)
  9. Creating Polyploid Escherichia Coli and Its Application in Efficient L-Threonine Production. Wang S, Chen X, Jin X, Gu F, Jiang W, Qi Q, Liang Q. Adv Sci (Weinh) 10 e2302417 (2023)
  10. Eukaryotic-like gephyrin and cognate membrane receptor coordinate corynebacterial cell division and polar elongation. Martinez M, Petit J, Leyva A, Sogues A, Megrian D, Rodriguez A, Gaday Q, Ben Assaya M, Portela MM, Haouz A, Ducret A, Grangeasse C, Alzari PM, Durán R, Wehenkel AM. Nat Microbiol 8 1896-1910 (2023)


Quick links