› 6se3

X-ray diffraction
2.8Å resolution

Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 3-6

Released:
DOI: 10.2210/pdb6se3/pdb
PDB entry 6se3 coloured by chain, front view.
PDB entry 6se3 coloured by chain, side view.
PDB entry 6se3 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.
Nicoll CR, Bailleul G, Fiorentini F, Mascotti ML, Fraaije MW, Mattevi A
Nat Struct Mol Biol 27 14-24 (2020)
PMID: 31873300

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ancestral Flavin-containing monooxygenase (FMO) 3-6 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 532 amino acids
Theoretical weight: 60.13 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand FAD 6 x FAD
Ligand NAP 6 x NAP
Ligand OXY 6 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 156.089Å b: 156.089Å c: 370.605Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.217 0.266
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi.
Ayuso-Fernández et al. (2022)
1 more

2 mentions without citation

Ancestral reconstruction of mammalian FMO1 enables structural determination, revealing unique features that explain its catalytic properties.
Bailleul et al. (2021)
1 more