› 6sop

X-ray diffraction
1.93Å resolution

Metal free structure of SynFtn variant E62A

Released:
DOI: 10.2210/pdb6sop/pdb
PDB entry 6sop coloured by chain, front view.
PDB entry 6sop coloured by chain, side view.
PDB entry 6sop coloured by chain, top view.
Source organism: Synechococcus sp. CC9311
Primary publication:
Routes of iron entry into, and exit from, the catalytic ferroxidase sites of the prokaryotic ferritin SynFtn.
Bradley JM, Pullin J, Moore GR, Svistunenko DA, Hemmings AM, Le Brun NE
Dalton Trans 49 1545-1554 (2020)
PMID: 31930254

Function and Biology Details

Reaction catalysed: 
(1a) 2 Fe(2+) + O(2) + 4 H(2)O = 2 (FeO(OH)) + 4 H(+) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170962 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.17 KDa
Source organism: Synechococcus sp. CC9311
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0I9X8 (Residues: 1-182; Coverage: 100%)
Gene name: sync_1539
Sequence domains: Ferritin-like domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: F432
Unit cell:
a: 176.89Å b: 176.89Å c: 176.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Bacterial iron detoxification at the molecular level.
Bradley et al. (2020)
1 more