› 6spc

Electron Microscopy
2.95Å resolution

Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate

Released:
DOI: 10.2210/pdb6spc/pdb
PDB entry 6spc coloured by chain, front view.
PDB entry 6spc coloured by chain, side view.
PDB entry 6spc coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of Pseudomonas aeruginosa ribosomes from an aminoglycoside-resistant clinical isolate.
Halfon Y, Jimenez-Fernandez A, La Rosa R, Espinosa Portero R, Krogh Johansen H, Matzov D, Eyal Z, Bashan A, Zimmerman E, Belousoff M, Molin S, Yonath A
OpenAccess logo Proc Natl Acad Sci U S A 116 22275-22281 (2019)
PMID: 31611393
Related structures: EMD-10281

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100413 (preferred)
Entry contents:
20 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (21 distinct):
Small ribosomal subunit protein uS2 Chain: b
Molecule details ›
Chain: b
Length: 221 amino acids
Theoretical weight: 24.76 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A072ZPV8 (Residues: 3-228; Coverage: 90%)
Gene names: CAZ10_16950, GNQ48_06145, GUL26_01875, IPC1295_18805, IPC737_19540, L4V69_10095, PAERUG_P19_London_7_VIM_2_05_10_03821, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: c
Molecule details ›
Chain: c
Length: 203 amino acids
Theoretical weight: 23.18 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A140S919 (Residues: 5-207; Coverage: 89%)
Gene names: CAZ10_34040, CSB93_1990, DT376_00190, GNQ48_26655, GUL26_34490, IPC1295_31605, IPC737_32970, L4V69_07070, PAERUG_P19_London_7_VIM_2_05_10_03305, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: d
Molecule details ›
Chain: d
Length: 205 amino acids
Theoretical weight: 23.19 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A072ZDF7 (Residues: 2-206; Coverage: 100%)
Gene names: CAZ10_34130, DT376_00280, GNQ48_26565, GUL26_34400, IPC1295_31515, IPC737_33060, L4V69_07160, PAERUG_P19_London_7_VIM_2_05_10_03323, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: e
Molecule details ›
Chain: e
Length: 149 amino acids
Theoretical weight: 15.69 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A241XG65 (Residues: 11-160; Coverage: 90%)
Gene names: CAZ10_34095, CSB93_2001, DT376_00245, GNQ48_26600, GUL26_34435, IPC1295_31550, IPC737_33025, L4V69_07125, PAERUG_P19_London_7_VIM_2_05_10_03316, rpsE
Sequence domains:
Small ribosomal subunit protein bS6 Chain: f
Molecule details ›
Chain: f
Length: 100 amino acids
Theoretical weight: 11.61 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A069Q263 (Residues: 2-101; Coverage: 72%)
Gene names: ALP65_00135, CAZ10_01825, CSB93_0497, DT376_23925, GNQ48_04620, GUL26_08725, IPC1295_07615, IPC737_06170, L4V69_33950, PAERUG_P19_London_7_VIM_2_05_10_02992, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: g
Molecule details ›
Chain: g
Length: 154 amino acids
Theoretical weight: 17.31 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A2V3F2U6 (Residues: 3-156; Coverage: 99%)
Gene names: CAZ10_33990, CSB93_1980, DT376_04900, GNQ48_26705, GUL26_34540, IPC1295_33155, IPC737_32915, L4V69_07020, PAERUG_P19_London_7_VIM_2_05_10_03295, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: h
Molecule details ›
Chain: h
Length: 125 amino acids
Theoretical weight: 13.54 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: E2RXT9 (Residues: 2-126; Coverage: 96%)
Gene names: CAZ10_34080, CSB93_1998, DT376_00230, GNQ48_26615, GUL26_34450, IPC1295_31565, IPC737_33010, L4V69_07110, PAERUG_P19_London_7_VIM_2_05_10_03313, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: i
Molecule details ›
Chain: i
Length: 126 amino acids
Theoretical weight: 14.14 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A069PXX1 (Residues: 3-128; Coverage: 97%)
Gene names: ALP65_00500, CAZ10_21155, DT376_14745, GNQ48_11360, GUL26_13450, IPC1295_15460, IPC737_20725, L4V69_30135, PAERUG_P19_London_7_VIM_2_05_10_00232, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: j
Molecule details ›
Chain: j
Length: 96 amino acids
Theoretical weight: 10.89 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: E2RXT0 (Residues: 8-103; Coverage: 93%)
Gene names: CAZ10_34005, CSB93_1983, DT376_00155, GNQ48_26690, GUL26_34525, IPC1295_31640, IPC737_32935, L4V69_07035, PAERUG_P19_London_7_VIM_2_05_10_03298, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: k
Molecule details ›
Chain: k
Length: 115 amino acids
Theoretical weight: 12.09 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: E2RXU4 (Residues: 14-128; Coverage: 89%)
Gene names: CAZ10_34125, CSB93_2007, DT376_00275, GNQ48_26570, GUL26_34405, IPC1295_31520, IPC737_33055, L4V69_07155, PAERUG_P19_London_7_VIM_2_05_10_03322, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: l
Molecule details ›
Chain: l
Length: 120 amino acids
Theoretical weight: 13.47 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A071L394 (Residues: 2-121; Coverage: 98%)
Gene names: CAZ10_33985, CSB93_1979, DT376_04905, GNQ48_26710, GUL26_34545, IPC1295_33150, IPC737_32910, L4V69_07015, PAERUG_P19_London_7_VIM_2_05_10_03294, rpsL
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: m
Molecule details ›
Chain: m
Length: 109 amino acids
Theoretical weight: 12.14 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: E2RXU3 (Residues: 3-112; Coverage: 92%)
Gene names: CAZ10_34120, DT376_00270, GNQ48_26575, GUL26_34410, IPC1295_31525, IPC737_33050, L4V69_07150, PAERUG_P19_London_7_VIM_2_05_10_03321, rpsM
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS14 Chain: n
Molecule details ›
Chain: n
Length: 98 amino acids
Theoretical weight: 11.2 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: E2RXT8 (Residues: 3-100; Coverage: 97%)
Gene names: CAZ10_34075, DT376_00225, GNQ48_26620, GUL26_34455, IPC1295_31570, IPC737_33005, L4V69_07105, PAERUG_P19_London_7_VIM_2_05_10_03312, rpsN
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: o
Molecule details ›
Chain: o
Length: 87 amino acids
Theoretical weight: 9.85 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A071L3R7 (Residues: 2-88; Coverage: 98%)
Gene names: ALP65_03471, CAZ10_02835, CSB93_0290, DT376_25075, GNQ48_21280, GUL26_09965, IPC1295_06610, IPC737_07210, L4V69_32945, PAERUG_P19_London_7_VIM_2_05_10_01502, rpsO
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: p
Molecule details ›
Chain: p
Length: 78 amino acids
Theoretical weight: 8.76 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A2V4FRZ2 (Residues: 1-78; Coverage: 94%)
Gene names: ALP65_02195, CAZ10_17400, CSB93_2527, DT376_21845, GUL26_01425, IPC1295_19255, L4V69_09645, PAERUG_P19_London_7_VIM_2_05_10_03417, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: q
Molecule details ›
Chain: q
Length: 76 amino acids
Theoretical weight: 8.85 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: E2RXT5 (Residues: 9-84; Coverage: 86%)
Gene names: CAZ10_34055, CSB93_1993, DT376_00205, GNQ48_26640, GUL26_34475, IPC1295_31590, IPC737_32985, L4V69_07085, PAERUG_P19_London_7_VIM_2_05_10_03308, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS18 Chain: r
Molecule details ›
Chain: r
Length: 56 amino acids
Theoretical weight: 6.41 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A2V3DLV3 (Residues: 19-74; Coverage: 74%)
Gene names: ALP65_100000, CAZ10_01830, CSB93_0496, DT376_23920, GNQ48_04625, GUL26_08730, IPC1295_07610, IPC737_06175, L4V69_33945, PAERUG_P19_London_7_VIM_2_05_10_02991, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein uS19 Chain: s
Molecule details ›
Chain: s
Length: 80 amino acids
Theoretical weight: 9.18 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: E2RXT2 (Residues: 2-81; Coverage: 88%)
Gene names: CAZ10_34030, CSB93_1988, DT376_00180, GNQ48_26665, GUL26_34500, IPC1295_31615, IPC737_32960, L4V69_07060, PAERUG_P19_London_7_VIM_2_05_10_03303, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein bS20 Chain: t
Molecule details ›
Chain: t
Length: 86 amino acids
Theoretical weight: 9.5 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A072ZDZ9 (Residues: 2-87; Coverage: 95%)
Gene names: ALP65_02387, DT376_27735, GNQ48_15755, GUL26_14600, IPC1295_27330, IPC737_30940, L4V69_31680, PAERUG_P19_London_7_VIM_2_05_10_04532, rpsT
Sequence domains: Ribosomal protein S20
Small ribosomal subunit protein bS21 Chain: u
Molecule details ›
Chain: u
Length: 34 amino acids
Theoretical weight: 4.21 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: A0A069QC99 (Residues: 34-67; Coverage: 48%)
Gene names: ALP65_01521, CAZ10_18885, CSB93_1880, DT376_28965, GNQ48_07015, GUL26_17630, IPC1295_08805, IPC737_10955, L4V69_06540, PAERUG_P19_London_7_VIM_2_05_10_01930, rpsU
Sequence domains: Ribosomal protein S21
16S ribosomal RNA Chain: a
Molecule details ›
Chain: a
Length: 1519 nucleotides
Theoretical weight: 492.39 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.95Å
Relevant EMDB volumes: EMD-10281

Quick links

Citations

4 review citations

Pseudomonas aeruginosa adaptation and evolution in patients with cystic fibrosis.
Rossi et al. (2021)
3 more

1 mention without citation

Structure of Pseudomonas aeruginosa ribosomes from an aminoglycoside-resistant clinical isolate.
Halfon et al. (2019)