› 6st1

X-ray diffraction
1.55Å resolution

Taurine ABC transporter substrate binding protein TauA from E. coli in complex with 2-(N-Morpholino)ethanesulfonic acid (MES)

Released:
DOI: 10.2210/pdb6st1/pdb
PDB entry 6st1 coloured by chain, front view.
PDB entry 6st1 coloured by chain, side view.
PDB entry 6st1 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Desolvation of the substrate-binding protein TauA dictates ligand specificity for the alkanesulfonate ABC importer TauABC.
Qu F, ElOmari K, Wagner A, De Simone A, Beis K
OpenAccess logo Biochem J 476 3649-3660 (2019)
PMID: 31802112

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Taurine-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 32.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47537 (Residues: 22-319; Coverage: 100%)
Gene names: JW0357, b0365, ssiA, tauA, yaiR
Sequence domains: Substrate binding domain of ABC-type glycine betaine transport system

Ligands and Environments

3 bound ligands:
Ligand MES 2 x MES
Ligand IOD 14 x IOD
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 45.22Å b: 73.02Å c: 163.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.231
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Franconibacter sp., 1MS: A New Strain in Decolorization and Degradation of Azo Dyes Ponceau S Red and Methyl Orange.
Baena-Baldiris et al. (2020)
7 more

1 mention without citation

Desolvation of the substrate-binding protein TauA dictates ligand specificity for the alkanesulfonate ABC importer TauABC.
Qu et al. (2019)