› 6sud

X-ray diffraction
1.74Å resolution

Structure of L320A mutant of Rex8A from Paenibacillus barcinonensis complexed with xylose.

Released:
DOI: 10.2210/pdb6sud/pdb
Model geometry
Fit model/data
PDB entry 6sud coloured by chain, front view.
PDB entry 6sud coloured by chain, side view.
PDB entry 6sud coloured by chain, top view.
Source organism: Paenibacillus barcinonensis
Primary publication:
Structural analysis of the reducing-end xylose-releasing exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 deciphers its molecular specificity.
Jiménez-Ortega E, Valenzuela S, Ramírez-Escudero M, Pastor FJ, Sanz-Aparicio J
FEBS J 287 5362-5374 (2020)
PMID: 32352213

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-xylose residues from the reducing end of oligosaccharides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reducing-end xylose-releasing exo-oligoxylanase Rex8A Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 44.48 KDa
Source organism: Paenibacillus barcinonensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0S2UQQ5 (Residues: 1-380; Coverage: 100%)
Gene names: DFQ00_11062, rex8A
Sequence domains: Glycosyl hydrolases family 8

Ligands and Environments

3 bound ligands:
Ligand EDO 12 x EDO
Ligand GOL 4 x GOL
Ligand XYP 3 x XYP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P1
Unit cell:
a: 51.514Å b: 59.172Å c: 79.412Å
α: 87.83° β: 78.35° γ: 74.61°
R-values:
R R work R free
0.244 0.242 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii.
Kmezik et al. (2021)
2 more

1 mention without citation

Structural analysis of the reducing-end xylose-releasing exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 deciphers its molecular specificity.
Jiménez-Ortega et al. (2020)

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO