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Cryo-EM study of an archaeal 30S initiation complex gives insights into evolution of translation initiation.

<div class='caption-title'>Cryo-EM maps of two conformations of the initiation complex 30S:mRNA:aIF1A:TC (IC2).</div>
<div class='caption-title'>Identified archaeal specificities of Pa-30S.</div>
<div class='caption-title'>Initiator tRNA interactions at the P site.</div>
Coureux PD, Lazennec-Schurdevin C, Bourcier S, Mechulam Y, Schmitt E
OpenAccess logo Commun Biol 3 58 (2020)
Related entries: 6swc, 6swd, 6swe

Cited: 19 times
EuropePMC logo PMID: 32029867

Abstract

Archaeal translation initiation occurs within a macromolecular complex containing the small ribosomal subunit (30S) bound to mRNA, initiation factors aIF1, aIF1A and the ternary complex aIF2:GDPNP:Met-tRNAiMet. Here, we determine the cryo-EM structure of a 30S:mRNA:aIF1A:aIF2:GTP:Met-tRNAiMet complex from Pyrococcus abyssi at 3.2 Å resolution. It highlights archaeal features in ribosomal proteins and rRNA modifications. We find an aS21 protein, at the location of eS21 in eukaryotic ribosomes. Moreover, we identify an N-terminal extension of archaeal eL41 contacting the P site. We characterize 34 N4-acetylcytidines distributed throughout 16S rRNA, likely contributing to hyperthermostability. Without aIF1, the 30S head is stabilized and initiator tRNA is tightly bound to the P site. A network of interactions involving tRNA, mRNA, rRNA modified nucleotides and C-terminal tails of uS9, uS13 and uS19 is observed. Universal features and domain-specific idiosyncrasies of translation initiation are discussed in light of ribosomal structures from representatives of each domain of life.

Articles - 6sw9 mentioned but not cited (2)

  1. Cryo-EM study of an archaeal 30S initiation complex gives insights into evolution of translation initiation. Coureux PD, Lazennec-Schurdevin C, Bourcier S, Mechulam Y, Schmitt E. Commun Biol 3 58 (2020)
  2. Coupling of Transcription and Translation in Archaea: Cues From the Bacterial World. Weixlbaumer A, Grünberger F, Werner F, Grohmann D. Front Microbiol 12 661827 (2021)


Reviews citing this publication (5)

  1. Recent Advances in Archaeal Translation Initiation. Schmitt E, Coureux PD, Kazan R, Bourgeois G, Lazennec-Schurdevin C, Mechulam Y. Front Microbiol 11 584152 (2020)
  2. Ribosome Biogenesis in Archaea. Londei P, Ferreira-Cerca S. Front Microbiol 12 686977 (2021)
  3. The diversity of Shine-Dalgarno sequences sheds light on the evolution of translation initiation. Wen JD, Kuo ST, Chou HD. RNA Biol 18 1489-1500 (2021)
  4. A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity Across the Tree of Life. Jüttner M, Ferreira-Cerca S. Methods Mol Biol 2533 3-22 (2022)
  5. Archaea/eukaryote-specific ribosomal proteins - guardians of a complex structure. Kisly I, Tamm T. Comput Struct Biotechnol J 21 1249-1261 (2023)

Articles citing this publication (12)



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