› 6sx0

X-ray diffraction
1.75Å resolution

Specific dsRNA recognition by wild type H7N1 NS1 RNA-binding domain

Released:
DOI: 10.2210/pdb6sx0/pdb
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PDB entry 6sx0 coloured by chain, front view.
PDB entry 6sx0 coloured by chain, side view.
PDB entry 6sx0 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and Sequence Determinants Governing the Interactions of RNAs with Influenza A Virus Non-Structural Protein NS1.
Wacquiez A, Coste F, Kut E, Gaudon V, Trapp S, Castaing B, Marc D
OpenAccess logo Viruses 12 (2020)
PMID: 32867106

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172992 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Non-structural protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 77 amino acids
Theoretical weight: 8.82 KDa
Source organism: Influenza A virus (A/turkey/Italy/977/1999(H7N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1PST0 (Residues: 2-73; Coverage: 31%)
Gene names: NS, NS1, ns1
Sequence domains: Influenza non-structural protein (NS1)
RNA (5'-R(*GP*GP*UP*AP*AP*CP*UP*GP*UP*UP*AP*CP*AP*GP*UP*UP*AP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 19 nucleotides
Theoretical weight: 6.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand PE4 5 x PE4
Ligand NHE 1 x NHE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 49.785Å b: 60.397Å c: 87.396Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.203
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

1 review citation

Structural Investigations of Interactions between the Influenza a Virus NS1 and Host Cellular Proteins.
Blake et al. (2023)
1 other article

1 mention without citation

Structure and Sequence Determinants Governing the Interactions of RNAs with Influenza A Virus Non-Structural Protein NS1.
Wacquiez et al. (2020)

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