› 6sz9

Electron Microscopy
3.7Å resolution

Type IV Coupling Complex (T4CC) from L. pneumophila.

Released:
DOI: 10.2210/pdb6sz9/pdb
PDB entry 6sz9 coloured by chain, front view.
PDB entry 6sz9 coloured by chain, side view.
PDB entry 6sz9 coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila.
Meir A, Macé K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G
OpenAccess logo Nat Commun 11 2864 (2020)
PMID: 32513920
Related structures: EMD-10350

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-178662 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Type 4 coupling protein DotL Chain: A
Molecule details ›
Chain: A
Length: 783 amino acids
Theoretical weight: 87.6 KDa
Source organism: Legionella pneumophila
Expression system: Legionella pneumophila
UniProt:
  • Canonical: Q5ZYC6 (Residues: 1-783; Coverage: 100%)
Gene names: dotL, icmO, lpg0446
Sequence domains: TraM recognition site of TraD and TraG
Type 4 apparatus protein DotM Chain: B
Molecule details ›
Chain: B
Length: 380 amino acids
Theoretical weight: 43.86 KDa
Source organism: Legionella pneumophila
Expression system: Legionella pneumophila
UniProt:
  • Canonical: Q5ZYC7 (Residues: 1-380; Coverage: 100%)
Gene names: dotM, icmP, lpg0445
Type 4 apparatus protein DotN Chain: C
Molecule details ›
Chain: C
Length: 208 amino acids
Theoretical weight: 23.76 KDa
Source organism: Legionella pneumophila
Expression system: Legionella pneumophila
UniProt:
  • Canonical: Q5ZYB7 (Residues: 7-214; Coverage: 97%)
Gene names: dotN, icmJ, lpg0455
Type 4 apparatus protein DotZ Chain: D
Molecule details ›
Chain: D
Length: 294 amino acids
Theoretical weight: 33.89 KDa
Source organism: Legionella pneumophila
Expression system: Legionella pneumophila
UniProt:
  • Canonical: Q5ZV91 (Residues: 1-294; Coverage: 100%)
Gene names: dotZ, lpg1549
Type 4 apparatus protein DotY Chain: E
Molecule details ›
Chain: E
Length: 230 amino acids
Theoretical weight: 25.55 KDa
Source organism: Legionella pneumophila
Expression system: Legionella pneumophila
UniProt:
  • Canonical: Q5ZYR7 (Residues: 1-230; Coverage: 100%)
Gene names: dotY, lpg0294

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-10350
Expression system: Legionella pneumophila

Quick links

Citations

9 review citations

Type IV secretion systems: Advances in structure, function, and activation.
Costa et al. (2021)
8 more

4 mentions without citation

Protein Dynamics in F-like Bacterial Conjugation.
Bragagnolo et al. (2020)
3 more