› 6t18

X-ray diffraction
3.15Å resolution

ASR Alternansucrase in complex with oligoalternan

Released:
DOI: 10.2210/pdb6t18/pdb
PDB entry 6t18 coloured by chain, front view.
PDB entry 6t18 coloured by chain, side view.
PDB entry 6t18 coloured by chain, top view.
Source organism: Leuconostoc mesenteroides
Primary publication:
A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.
Molina M, Moulis C, Monties N, Guieysse D, Morel S, Cioci G, Remaud-Siméon M
J Biol Chem 295 9474-9489 (2020)
PMID: 32409580

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
dextransucrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 1278 amino acids
Theoretical weight: 142.58 KDa
Source organism: Leuconostoc mesenteroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RE05 (Residues: 147-1424; Coverage: 63%)
Gene name: asr
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 100.876Å b: 135.654Å c: 239.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.226
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure-Function Relationship Studies and Engineering.
Molina et al. (2021)

1 mention without citation

A specific oligosaccharide-binding site in the alternansucrase catalytic domain mediates alternan elongation.
Molina et al. (2020)