› 6t32

X-ray diffraction
1.75Å resolution

Streptavidin variants harbouring an artificial organocatalyst based cofactor

Released:
DOI: 10.2210/pdb6t32/pdb
PDB entry 6t32 coloured by chain, front view.
PDB entry 6t32 coloured by chain, side view.
PDB entry 6t32 coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
An Artificial Cofactor Catalyzing the Baylis-Hillman Reaction with Designed Streptavidin as Protein Host*.
Lechner H, Emann VR, Breuning M, Höcker B
OpenAccess logo Chembiochem 22 1573-1577 (2021)
PMID: 33400831

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 16.53 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-183; Coverage: 92%)
Sequence domains: Avidin family

Ligands and Environments

1 bound ligand:
Ligand HL9 2 x HL9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 57.33Å b: 85.085Å c: 99.444Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.191
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Derivatives of Natural Organocatalytic Cofactors and Artificial Organocatalytic Cofactors as Catalysts in Enzymes.
Lechner et al. (2022)
2 other articles

1 mention without citation

An Artificial Cofactor Catalyzing the Baylis-Hillman Reaction with Designed Streptavidin as Protein Host*.
Lechner et al. (2021)