› 6t58

X-ray diffraction
3.1Å resolution

Structure determination of the transactivation domain of p53 in complex with S100A4 using annexin A2 as a crystallization chaperone

Released:
DOI: 10.2210/pdb6t58/pdb
PDB entry 6t58 coloured by chain, front view.
PDB entry 6t58 coloured by chain, side view.
PDB entry 6t58 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure Determination of the Transactivation Domain of p53 in Complex with S100A4 Using Annexin A2 as a Crystallization Chaperone.
Ecsédi P, Gógl G, Hóf H, Kiss B, Harmat V, Nyitray L
Structure 28 943-953.e4 (2020)
PMID: 32442400

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138101 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Annexin A2; Cellular tumor antigen p53; Protein S100-A4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 553 amino acids
Theoretical weight: 63.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 17-56; Coverage: 10%)
  • Canonical: P26447 (Residues: 1-93; Coverage: 92%)
  • Canonical: P07355 (Residues: 25-26, 29-339; Coverage: 92%)
Gene names: ANX2, ANX2L4, ANXA2, CAL1H, CAPL, LPC2D, MTS1, P53, S100A4, TP53
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand CA 16 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 95.96Å b: 62.77Å c: 106.02Å
α: 90° β: 90.26° γ: 90°
R-values:
R R work R free
0.234 0.23 0.272
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Targeting p53 pathways: mechanisms, structures, and advances in therapy.
Wang et al. (2023)
3 more

2 mentions without citation

Studying the Structures of Relaxed and Fuzzy Interactions: The Diverse World of S100 Complexes.
Ecsédi et al. (2021)
1 more