› 6t5k

X-ray diffraction
1.33Å resolution

ECV-1 from Echinicola vietnamensis. Environmental metallo-beta-lactamases exhibit high enzymatic activity under zinc deprivation

Released:
DOI: 10.2210/pdb6t5k/pdb
PDB entry 6t5k coloured by chain, front view.
PDB entry 6t5k coloured by chain, side view.
PDB entry 6t5k coloured by chain, top view.
Source organism: Echinicola vietnamensis
Primary publication:
Structural and biochemical characterization of the environmental MBLs MYO-1, ECV-1 and SHD-1.
Fröhlich C, Sørum V, Huber S, Samuelsen Ø, Berglund F, Kristiansson E, Kotsakis SD, Marathe NP, Larsson DGJ, Leiros HS
OpenAccess logo J Antimicrob Chemother 75 2554-2563 (2020)
PMID: 32464640

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chain: C
Molecule details ›
Chain: C
Length: 240 amino acids
Theoretical weight: 26.39 KDa
Source organism: Echinicola vietnamensis
Expression system: Escherichia coli
UniProt:
  • Canonical: L0FY79 (Residues: 18-257; Coverage: 93%)
Gene name: Echvi_2632
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand EDO 5 x EDO
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: C2221
Unit cell:
a: 51.926Å b: 65.676Å c: 128.501Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.184
Expression system: Escherichia coli

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Citations

1 review citation

Comparative genomic assessment of members of genus Tenacibaculum: an exploratory study.
Satyam et al. (2023)
3 other articles

2 mentions without citation

Structural and biochemical characterization of the environmental MBLs MYO-1, ECV-1 and SHD-1.
Fröhlich et al. (2020)
1 more