› 6t5z

X-ray diffraction
1.6Å resolution

Crystal structure of an AA10 LPMO from Photorhabdus luminescens

Released:
DOI: 10.2210/pdb6t5z/pdb
PDB entry 6t5z coloured by chain, front view.
PDB entry 6t5z coloured by chain, side view.
PDB entry 6t5z coloured by chain, top view.
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Primary publication:
Characterization of a bacterial copper-dependent lytic polysaccharide monooxygenase with an unusual second coordination sphere.
Munzone A, El Kerdi B, Fanuel M, Rogniaux H, Ropartz D, Réglier M, Royant A, Simaan AJ, Decroos C
FEBS J 287 3298-3314 (2020)
PMID: 31903721

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chitin-binding type-4 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 174 amino acids
Theoretical weight: 19.6 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7N4I5 (Residues: 26-199; Coverage: 100%)
Gene name: plu2352
Sequence domains: Lytic polysaccharide mono-oxygenase, cellulose-degrading

Ligands and Environments

1 bound ligand:
Ligand CU 6 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 76.53Å b: 80.39Å c: 90.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.218
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review.
Sethupathy et al. (2021)
1 more

3 mentions without citation

Characterization of a bacterial copper-dependent lytic polysaccharide monooxygenase with an unusual second coordination sphere.
Munzone et al. (2020)
2 more