Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Proliferating cell nuclear antigen, PCNA
- DNA clamp superfamily
- DNA polymerase II large subunit DP2
- Proliferating cell nuclear antigen, PCNA, C-terminal
- Proliferating cell nuclear antigen, PCNA, conserved site
- Proliferating cell nuclear antigen, PCNA, N-terminal
- Nucleic acid-binding, OB-fold
- DNA polymerase II small subunit, archaeal
4 more domains
Structure analysis Details
Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-120637 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
DNA polymerase sliding clamp
Molecule details ›
Chains: C, D, E
Length: 261 amino acids
Theoretical weight: 29.47 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 261 amino acids
Theoretical weight: 29.47 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli
UniProt:
- Canonical:
Q9UYX8 (Residues: 1-249; Coverage: 100%)
Sequence domains:
DNA polymerase II small subunit
Molecule details ›
Chain: A
Length: 619 amino acids
Theoretical weight: 69.41 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 619 amino acids
Theoretical weight: 69.41 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli
UniProt:
- Canonical: Q9V2F3 (Residues: 1-619; Coverage: 100%)
Sequence domains:
DNA polymerase II large subunit, 1st part
Molecule details ›
Chain: B
Length: 1275 amino acids
Theoretical weight: 144.76 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli
UniProt:
Sequence domains: DNA polymerase II large subunit DP2
Length: 1275 amino acids
Theoretical weight: 144.76 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli
UniProt:
- Canonical: Q9V2F4 (Residues: 2-1455; Coverage: 87%)
Sequence domains: DNA polymerase II large subunit DP2
