› 6t9w

X-ray diffraction
2.15Å resolution

Crystal structure of formate dehydrogenase FDH2 D222A/Q223R enzyme from Granulicella mallensis MP5ACTX8 in complex with NADP and azide.

Released:
DOI: 10.2210/pdb6t9w/pdb
PDB entry 6t9w coloured by chain, front view.
PDB entry 6t9w coloured by chain, side view.
PDB entry 6t9w coloured by chain, top view.
Source organism: Granulicella mallensis MP5ACTX8
Primary publication:
From the Amelioration of a NADP+-dependent Formate Dehydrogenase to the Discovery of a New Enzyme: Round Trip from Theory to Practice
Robescu MS, Rubini R, Beneventi E, Tavanti M, Lonigro C, Zito F, Filippini F, Cendron L, Bergantino E
ChemCatChem (2020)

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-515597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 386 amino acids
Theoretical weight: 42.57 KDa
Source organism: Granulicella mallensis MP5ACTX8
Expression system: Escherichia coli
UniProt:
  • Canonical: G8NTI5 (Residues: 1-386; Coverage: 100%)
Gene name: AciX8_0868
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NAP 3 x NAP
Ligand AZI 3 x AZI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: C2
Unit cell:
a: 126.53Å b: 62.08Å c: 211.745Å
α: 90° β: 91.25° γ: 90°
R-values:
R R work R free
0.191 0.191 0.231
Expression system: Escherichia coli

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