› 6tc0

X-ray diffraction
3.6Å resolution

Crystal structure of MMS19-CIAO1-CIAO2B CIA targeting complex

Released:
DOI: 10.2210/pdb6tc0/pdb
Model geometry
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PDB entry 6tc0 coloured by chain, front view.
PDB entry 6tc0 coloured by chain, side view.
PDB entry 6tc0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into Fe-S protein biogenesis by the CIA targeting complex.
Kassube SA, Thomä NH
Nat Struct Mol Biol 27 735-742 (2020)
PMID: 32632277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-181532 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Probable cytosolic iron-sulfur protein assembly protein Ciao1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 338 amino acids
Theoretical weight: 37.28 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q7K1Y4 (Residues: 2-335; Coverage: 100%)
Gene names: CG12797, Ciao1
Sequence domains: WD domain, G-beta repeat
MIP18 family protein galla-2 Chains: B, E
Molecule details ›
Chains: B, E
Length: 159 amino acids
Theoretical weight: 17.81 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VTC4 (Residues: 2-156; Coverage: 99%)
Gene names: CG7949, galla-2
Sequence domains: Iron-sulfur cluster assembly protein
MMS19 nucleotide excision repair protein homolog Chains: C, F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 107.4Å b: 140.7Å c: 327.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.258 0.277
Expression system: Trichoplusia ni

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Citations

3 review citations

Biogenesis of Iron-Sulfur Clusters and Their Role in DNA Metabolism.
Shi et al. (2021)
2 more

2 mentions without citation

Cytosolic iron-sulfur protein assembly system identifies clients by a C-terminal tripeptide.
Marquez et al. (2023)
1 more

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