› 6th5

X-ray diffraction
1.99Å resolution

Crystal structure of mature wildtype primitive Phytochelatin synthase from Nostoc spec. - Alr0975

Released:
DOI: 10.2210/pdb6th5/pdb
PDB entry 6th5 coloured by chain, front view.
PDB entry 6th5 coloured by chain, side view.
PDB entry 6th5 coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry authors: Feiler CG, Blankenfeldt W

Function and Biology Details

Reaction catalysed: 
Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutathione gamma-glutamylcysteinyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 24.64 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YY76 (Residues: 25-242; Coverage: 100%)
Gene name: alr0975
Sequence domains: Phytochelatin synthase

Ligands and Environments

1 bound ligand:
Ligand CA 5 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 61.356Å b: 46.67Å c: 68.583Å
α: 90° β: 92.258° γ: 90°
R-values:
R R work R free
0.201 0.199 0.238
Expression system: Escherichia coli BL21(DE3)

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