Structure analysis

Cryo-EM Structure of T. kodakarensis 70S ribosome

Electron Microscopy
2.55Å resolution
Assembly composition:
hetero 63-mer (preferred)
Entry contents: 58 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 63-mer
Accessible surface area: 841865.95 Å2
Buried surface area: 387888.38 Å2
Dissociation area: 564.59 Å2
Dissociation energy (ΔGdiss): -0.72 kcal/mol
Dissociation entropy (TΔSdiss): 12.55 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-250348

Macromolecules

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Chain: Ad
Length: 200 amino acids
Theoretical weight: 23.06 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JGM4 (Residues: 1-200; Coverage: 100%)
Gene names: TK1254, rps3ae
Pfam: Ribosomal S3Ae family
InterPro:

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Chain: Ah
Length: 125 amino acids
Theoretical weight: 13.76 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JDK8 (Residues: 1-125; Coverage: 100%)
Gene names: TK1951, rps6e
Pfam: Ribosomal protein S6e
InterPro:

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Chain: Aj
Length: 130 amino acids
Theoretical weight: 14.6 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JJG3 (Residues: 1-130; Coverage: 100%)
Gene names: TK1526, rps8
Pfam: Ribosomal protein S8
InterPro:

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Chain: Ak
Length: 130 amino acids
Theoretical weight: 14.55 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JGF3 (Residues: 1-130; Coverage: 100%)
Gene names: TK1191, rps8e
Pfam: Ribosomal protein S8e
InterPro:

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Chain: An
Length: 140 amino acids
Theoretical weight: 15.11 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JJF3 (Residues: 1-140; Coverage: 100%)
Gene names: TK1504, rps11
Pfam: Ribosomal protein S11
InterPro:

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Chain: At
Length: 67 amino acids
Theoretical weight: 8.04 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JDC7 (Residues: 1-67; Coverage: 100%)
Gene names: TK2292, rps17e
Pfam: Ribosomal S17
InterPro:

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Chain: Ax
Length: 65 amino acids
Theoretical weight: 7.07 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JE50 (Residues: 1-65; Coverage: 100%)
Gene names: TK1099, rps27e
Pfam: Ribosomal protein S27
InterPro:

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Chain: Ay
Length: 70 amino acids
Theoretical weight: 7.97 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JGR4 (Residues: 1-70; Coverage: 100%)
Gene names: TK1310, rps28e
Pfam: Ribosomal protein S28e
InterPro:

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Chain: BL
Length: 141 amino acids
Theoretical weight: 15.18 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JJF8 (Residues: 1-141; Coverage: 100%)
Gene names: TK1531, rpl14
Pfam: Ribosomal protein L14p/L23e
InterPro:

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Chain: BQ
Length: 201 amino acids
Theoretical weight: 22.9 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JJG7 (Residues: 1-201; Coverage: 100%)
Gene names: TK1522, rpl18
Pfam: Ribosomal large subunit proteins 60S L5, and 50S L18
InterPro: Large ribosomal subunit protein uL18 eukaryotic/archaeal

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Chain: BT
Length: 77 amino acids
Theoretical weight: 9.23 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JGT3 (Residues: 1-77; Coverage: 100%)
Gene names: TK1322, rpl18a, rpl20e, rplX
Pfam: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
InterPro:

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Chain: BZ
Length: 66 amino acids
Theoretical weight: 7.94 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JDH6 (Residues: 1-66; Coverage: 100%)
Gene names: TK1535, rpl29
Pfam: Ribosomal L29 protein
InterPro:

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Chain: Bc
Length: 90 amino acids
Theoretical weight: 10.35 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JGT5 (Residues: 1-90; Coverage: 100%)
Gene names: TK1320, rpl31e
Pfam: Ribosomal protein L31e
InterPro:

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Chain: Be
Length: 90 amino acids
Theoretical weight: 10.56 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JJF7 (Residues: 1-90; Coverage: 100%)
Gene names: TK1515, rpl34e
Pfam: Ribosomal protein L34e
InterPro:

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Chain: Bg
Length: 86 amino acids
Theoretical weight: 9.24 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JDM6 (Residues: 1-86; Coverage: 100%)
Gene names: TK0615, rpl37ae
Pfam: Ribosomal L37ae protein family
InterPro:

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Chain: Bk
Length: 37 amino acids
Theoretical weight: 5.05 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JEV0 (Residues: 1-37; Coverage: 100%)
Gene name: TK1915

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Chain: Bl
Length: 94 amino acids
Theoretical weight: 11.2 KDa
Source organism: Thermococcus kodakarensis KOD1
UniProt:
  • Canonical: Q5JE51 (Residues: 1-94; Coverage: 100%)
Gene names: TK1098, rpl44e
Pfam: Ribosomal protein L44
InterPro:

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Name: 16S ribosomal RNA
Representative chains: Aa
Length: 1498 nucleotides
Theoretical weight: 486.94 KDa
Rfam: Archaeal small subunit ribosomal RNA

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Name: 23S ribosomal RNA
Representative chains: BA
Length: 3037 nucleotides
Theoretical weight: 987.67 KDa
Rfam: Archaeal large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: BB
Length: 126 nucleotides
Theoretical weight: 40.74 KDa
Rfam: 5S ribosomal RNA

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