› 6thk

X-ray diffraction
2.2Å resolution

Structural mechanism of pyocin S5 import into Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb6thk/pdb
PDB entry 6thk coloured by chain, front view.
PDB entry 6thk coloured by chain, side view.
PDB entry 6thk coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Pyocin S5 Import into Pseudomonas aeruginosa Reveals a Generic Mode of Bacteriocin Transport.
Behrens HM, Lowe ED, Gault J, Housden NG, Kaminska R, Weber TM, Thompson CMA, Mislin GLA, Schalk IJ, Walker D, Robinson CV, Kleanthous C
OpenAccess logo mBio 11 (2020)
PMID: 32156826

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-575155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Channel forming colicins domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 506 amino acids
Theoretical weight: 57.22 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I4Y4 (Residues: 1-498; Coverage: 100%)
Gene names: PA0985, pyoS5
Sequence domains: Colicin pore forming domain

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 50.24Å b: 53.87Å c: 104.8Å
α: 90° β: 95.15° γ: 90°
R-values:
R R work R free
0.227 0.225 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Ton motor complexes.
Ratliff et al. (2021)
3 more

4 mentions without citation

Perspectives in Searching Antimicrobial Peptides (AMPs) Produced by the Microbiota.
Gallardo-Becerra et al. (2023)
3 more