› 6ti2

X-ray diffraction
2.75Å resolution

Structure of the Ustilago maydis chorismate mutase 1 in complex with KWL1-b from Zea mays

Released:
DOI: 10.2210/pdb6ti2/pdb
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PDB entry 6ti2 coloured by chain, front view.
PDB entry 6ti2 coloured by chain, side view.
PDB entry 6ti2 coloured by chain, top view.
Source organisms:
Primary publication:
The two paralogous kiwellin proteins KWL1 and KWL1-b from maize are structurally related and have overlapping functions in plant defense.
Altegoer F, Weiland P, Giammarinaro PI, Freibert SA, Binnebesel L, Han X, Lepak A, Kahmann R, Lechner M, Bange G
J Biol Chem 295 7816-7825 (2020)
PMID: 32350112

Function and Biology Details

Reaction catalysed: 
Chorismate = prephenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101096 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Secreted chorismate mutase Chains: B, C
Molecule details ›
Chains: B, C
Length: 278 amino acids
Theoretical weight: 31.06 KDa
Source organism: Ustilago maydis 521
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0D1DWQ2 (Residues: 22-290; Coverage: 100%)
Gene names: CMU1, UMAG_05731
Ripening-related protein 3 Chains: A, E
Molecule details ›
Chains: A, E
Length: 199 amino acids
Theoretical weight: 21.26 KDa
Source organism: Zea mays
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K7U7F7 (Residues: 1-193; Coverage: 100%)
Gene name: ZEAMMB73_Zm00001d026470

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 57.74Å b: 124.58Å c: 98.21Å
α: 90° β: 96.216° γ: 90°
R-values:
R R work R free
0.226 0.224 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Structural and functional analysis of the cerato-platanin-like protein Cpl1 suggests diverging functions in smut fungi.
Weiland et al. (2023)
2 more

2 mentions without citation

The two paralogous kiwellin proteins KWL1 and KWL1-b from maize are structurally related and have overlapping functions in plant defense.
Altegoer et al. (2020)
1 more

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