› 6tiy

X-ray diffraction
2.29Å resolution

DROSOPHILA GMPCPP-TUBULIN

Released:
DOI: 10.2210/pdb6tiy/pdb
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PDB entry 6tiy coloured by chain, front view.
PDB entry 6tiy coloured by chain, side view.
PDB entry 6tiy coloured by chain, top view.
Source organisms:
Primary publication:
GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.
Ayukawa R, Iwata S, Imai H, Kamimura S, Hayashi M, Ngo KX, Minoura I, Uchimura S, Makino T, Shirouzu M, Shigematsu H, Sekimoto K, Gigant B, Muto E
OpenAccess logo J Cell Biol 220 (2021)
PMID: 33544140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-139089 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1 chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 450 amino acids
Theoretical weight: 49.99 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P06603 (Residues: 1-450; Coverage: 100%)
Gene names: CG1913, alphaTub84B, tubA84B
Sequence domains:
Tubulin beta-1 chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 447 amino acids
Theoretical weight: 50.19 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24560 (Residues: 1-447; Coverage: 100%)
Gene names: CG9277, betaTub56D
Sequence domains:
Stathmin-4 Chain: E

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 4 x MG
Ligand SO4 11 x SO4
Ligand GOL 1 x GOL
Ligand G2P 2 x G2P
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 66.669Å b: 126.564Å c: 251.012Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.217
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

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Citations

4 review citations

Regulation of microtubule dynamics, mechanics and function through the growing tip.
Gudimchuk et al. (2021)
3 more

3 mentions without citation

GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.
Ayukawa et al. (2021)
2 more

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