› 6tiy

X-ray diffraction
2.29Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-139089 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1 chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 450 amino acids
Theoretical weight: 49.99 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P06603 (Residues: 1-450; Coverage: 100%)
Gene names: CG1913, alphaTub84B, tubA84B
Sequence domains:
Tubulin beta-1 chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 447 amino acids
Theoretical weight: 50.19 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q24560 (Residues: 1-447; Coverage: 100%)
Gene names: CG9277, betaTub56D
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 16.85 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 47-189; Coverage: 76%)
Gene name: Stmn4
Sequence domains: Stathmin family

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 4 x MG
Ligand SO4 11 x SO4
Ligand GOL 1 x GOL
Ligand G2P 2 x G2P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 66.669Å b: 126.564Å c: 251.012Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.217
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

4 review citations

Regulation of microtubule dynamics, mechanics and function through the growing tip.
Gudimchuk et al. (2021)
3 more

3 mentions without citation

GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.
Ayukawa et al. (2021)
2 more