› 6tjd

X-ray diffraction
2.1Å resolution

Crystal structure of the computationally designed Cake4 protein

Released:
DOI: 10.2210/pdb6tjd/pdb
PDB entry 6tjd coloured by chain, front view.
PDB entry 6tjd coloured by chain, side view.
PDB entry 6tjd coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural plasticity of a designer protein sheds light on β-propeller protein evolution.
Mylemans B, Laier I, Kamata K, Akashi S, Noguchi H, Tame JRH, Voet ARD
FEBS J 288 530-545 (2021)
PMID: 32343866

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167516 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cake4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 17.61 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 34.913Å b: 65.758Å c: 71.205Å
α: 90° β: 91.045° γ: 90°
R-values:
R R work R free
0.179 0.176 0.219
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development and applications of artificial symmetrical proteins.
Vrancken et al. (2020)
5 other articles

1 mention without citation

Structural plasticity of a designer protein sheds light on β-propeller protein evolution.
Mylemans et al. (2021)