Function and Biology

Cryo-EM structure of the Anaphase-promoting complex/Cyclosome, in complex with the Mitotic checkpoint complex (APC/C-MCC) at 3.8 angstrom resolution

Source organism: Homo sapiens
Biochemical function: protein binding
Biological process: cell division
Cellular component: cytosol

EC 2.7.11.1: Non-specific serine/threonine protein kinase

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Systematic name:
ATP:protein phosphotransferase (non-specific)
Alternative Name(s):
  • A-kinase
  • AP50 kinase
  • ATP-protein transphosphorylase
  • Beta-IIPKC
  • Beta-adrenergic receptor kinase
  • CK-2
  • CKI
  • CKII
  • Calcium-dependent protein kinase C
  • Calcium/phospholipid-dependent protein kinase
  • Casein kinase
  • Casein kinase (phosphorylating)
  • Casein kinase 2
  • Casein kinase I
  • Casein kinase II
  • Cyclic AMP-dependent protein kinase
  • Cyclic AMP-dependent protein kinase A
  • Cyclic monophosphate-dependent protein kinase
  • Cyclic nucleotide-dependent protein kinase
  • Cyclin-dependent kinase
  • Cytidine 3',5'-cyclic monophosphate-responsive protein kinase
  • Dsk1
  • Epsilon PKC
  • Glycogen synthase A kinase
  • Glycogen synthase a kinase
  • Glycogen synthase kinase
  • HIPK2
  • Hpr kinase
  • Hydroxyalkyl-protein kinase
  • M phase-specific cdc2 kinase
  • MAPK
  • Mitogen-activated S6 kinase
  • Mitogen-activated protein kinase
  • P82 kinase
  • PKA
  • PKC
  • Phosphorylase B kinase kinase
  • Phosphorylase b kinase kinase
  • Phosphorylase b kinase kinase
  • Protein glutamyl kinase
  • Protein kinase (phosphorylating)
  • Protein kinase A
  • Protein kinase CK2
  • Protein kinase p58
  • Protein phosphokinase
  • Protein serine kinase
  • Protein serine-threonine kinase
  • Protein-aspartyl kinase
  • Protein-cysteine kinase
  • Protein-serine kinase
  • Prp4 protein kinase
  • Raf kinase
  • Raf-1
  • Ribosomal S6 protein kinase
  • Ribosomal protein S6 kinase II
  • STK32
  • Serine kinase
  • Serine protein kinase
  • Serine(threonine) protein kinase
  • Serine-specific protein kinase
  • Serine/threonine protein kinase
  • T-antigen kinase
  • Threonine-specific protein kinase
  • Twitchin kinase
  • Type-2 casein kinase
  • WEE1Hu
  • Wee 1-like kinase
  • Wee-kinase
  • cAMP-dependent protein kinase
  • cAMP-dependent protein kinase A
  • cGMP-dependent protein kinase

GO terms

Biochemical function:
Biological process:
Cellular component:

Sequence families

Pfam Protein families (Pfam)
PF00400
Domain description: WD domain, G-beta repeat
Occurring in:
  1. Cell division cycle protein 20 homolog
  2. Cell division cycle protein 20 homolog
The deposited structure of PDB entry 6tlj contains 3 copies of Pfam domain PF00400 (WD domain, G-beta repeat) in Cell division cycle protein 20 homolog. Showing 3 copies in chain Q.
The deposited structure of PDB entry 6tlj contains 3 copies of Pfam domain PF00400 (WD domain, G-beta repeat) in Cell division cycle protein 20 homolog. Showing 3 copies in chain R.

PF00515
Domain description: Tetratricopeptide repeat
Occurring in:
  1. Cell division cycle protein 27 homolog
The deposited structure of PDB entry 6tlj contains 4 copies of Pfam domain PF00515 (Tetratricopeptide repeat) in Cell division cycle protein 27 homolog. Showing 2 copies in chain F.

PF12895
Domain description: Anaphase-promoting complex, cyclosome, subunit 3
Occurring in:
  1. Cell division cycle protein 27 homolog
  2. Cell division cycle protein 16 homolog
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF12895 (Anaphase-promoting complex, cyclosome, subunit 3) in Cell division cycle protein 27 homolog. Showing 1 copy in chain F.
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF12895 (Anaphase-promoting complex, cyclosome, subunit 3) in Cell division cycle protein 16 homolog. Showing 1 copy in chain J.

PF12894
Domain description: Anaphase-promoting complex subunit 4 WD40 domain
Occurring in:
  1. Anaphase-promoting complex subunit 4
  2. Cell division cycle protein 20 homolog
  3. Cell division cycle protein 20 homolog
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF12894 (Anaphase-promoting complex subunit 4 WD40 domain) in Anaphase-promoting complex subunit 4. Showing 1 copy in chain I.
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF12894 (Anaphase-promoting complex subunit 4 WD40 domain) in Cell division cycle protein 20 homolog. Showing 1 copy in chain Q.
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF12894 (Anaphase-promoting complex subunit 4 WD40 domain) in Cell division cycle protein 20 homolog. Showing 1 copy in chain R.

PF13432
Domain description: Tetratricopeptide repeat
Occurring in:
  1. Anaphase-promoting complex subunit 7
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF13432 (Tetratricopeptide repeat) in Anaphase-promoting complex subunit 7. Showing 1 copy in chain V [auth Y].

PF13181
Domain description: Tetratricopeptide repeat
Occurring in:
  1. Anaphase-promoting complex subunit 7
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF13181 (Tetratricopeptide repeat) in Anaphase-promoting complex subunit 7. Showing 1 copy in chain V [auth Y].

PF12862
Domain description: Anaphase-promoting complex subunit 5
Occurring in:
  1. Anaphase-promoting complex subunit 5
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF12862 (Anaphase-promoting complex subunit 5) in Anaphase-promoting complex subunit 5. Showing 2 copies in chain O.

PF13424
Domain description: Tetratricopeptide repeat
Occurring in:
  1. Cell division cycle protein 16 homolog
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF13424 (Tetratricopeptide repeat) in Cell division cycle protein 16 homolog. Showing 1 copy in chain J.

PF10471
Domain description: Anaphase-promoting complex APC subunit CDC26
Occurring in:
  1. Anaphase-promoting complex subunit CDC26
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF10471 (Anaphase-promoting complex APC subunit CDC26) in Anaphase-promoting complex subunit CDC26. Showing 1 copy in chain G.

PF04049
Domain description: Anaphase promoting complex subunit 8 / Cdc23
Occurring in:
  1. Cell division cycle protein 23 homolog
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF04049 (Anaphase promoting complex subunit 8 / Cdc23 ) in Cell division cycle protein 23 homolog. Showing 1 copy in chain C.

PF12859
Domain description: Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
Occurring in:
  1. Anaphase-promoting complex subunit 1
The deposited structure of PDB entry 6tlj contains 2 copies of Pfam domain PF12859 (Anaphase-promoting complex subunit 1 WD40 beta-propeller domain) in Anaphase-promoting complex subunit 1. Showing 2 copies in chain A.

PF02301
Domain description: HORMA domain
Occurring in:
  1. Mitotic spindle assembly checkpoint protein MAD2A
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF02301 (HORMA domain) in Mitotic spindle assembly checkpoint protein MAD2A. Showing 1 copy in chain W [auth Z].

PF08311
Domain description: Mad3/BUB1 homology region 1
Occurring in:
  1. Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF08311 (Mad3/BUB1 homology region 1) in Mitotic checkpoint serine/threonine-protein kinase BUB1 beta. Showing 1 copy in chain S.

PF21371
Domain description: Anaphase-promoting complex subunit 5, N-terminal domain
Occurring in:
  1. Anaphase-promoting complex subunit 5
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF21371 (Anaphase-promoting complex subunit 5, N-terminal domain) in Anaphase-promoting complex subunit 5. Showing 1 copy in chain O.

PF08672
Domain description: Anaphase promoting complex (APC) subunit 2
Occurring in:
  1. Anaphase-promoting complex subunit 2
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF08672 (Anaphase promoting complex (APC) subunit 2) in Anaphase-promoting complex subunit 2. Showing 1 copy in chain N.

PF00888
Domain description: Cullin family
Occurring in:
  1. Anaphase-promoting complex subunit 2
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF00888 (Cullin family) in Anaphase-promoting complex subunit 2. Showing 1 copy in chain N.

PF05839
Domain description: Apc13p protein
Occurring in:
  1. Anaphase-promoting complex subunit 13
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF05839 (Apc13p protein) in Anaphase-promoting complex subunit 13. Showing 1 copy in chain M.

PF03256
Domain description: Anaphase-promoting complex, subunit 10 (APC10)
Occurring in:
  1. Anaphase-promoting complex subunit 10
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF03256 (Anaphase-promoting complex, subunit 10 (APC10)) in Anaphase-promoting complex subunit 10. Showing 1 copy in chain L.

PF12896
Domain description: Anaphase-promoting complex, cyclosome, subunit 4
Occurring in:
  1. Anaphase-promoting complex subunit 4
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF12896 (Anaphase-promoting complex, cyclosome, subunit 4) in Anaphase-promoting complex subunit 4. Showing 1 copy in chain I.

PF17256
Domain description: Anaphase-promoting complex, subunit 16
Occurring in:
  1. Anaphase-promoting complex subunit 16
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF17256 (Anaphase-promoting complex, subunit 16) in Anaphase-promoting complex subunit 16. Showing 1 copy in chain E.

PF15243
Domain description: Anaphase-promoting complex subunit 15
Occurring in:
  1. Anaphase-promoting complex subunit 15
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF15243 (Anaphase-promoting complex subunit 15) in Anaphase-promoting complex subunit 15. Showing 1 copy in chain D.

PF12861
Domain description: Anaphase-promoting complex subunit 11 RING-H2 finger
Occurring in:
  1. Anaphase-promoting complex subunit 11
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF12861 (Anaphase-promoting complex subunit 11 RING-H2 finger) in Anaphase-promoting complex subunit 11. Showing 1 copy in chain B.

PF18122
Domain description: Anaphase-promoting complex sub unit 1 C-terminal domain
Occurring in:
  1. Anaphase-promoting complex subunit 1
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF18122 (Anaphase-promoting complex sub unit 1 C-terminal domain ) in Anaphase-promoting complex subunit 1. Showing 1 copy in chain A.

PF21282
Domain description: APC1 beta sandwich domain
Occurring in:
  1. Anaphase-promoting complex subunit 1
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF21282 (APC1 beta sandwich domain) in Anaphase-promoting complex subunit 1. Showing 1 copy in chain A.

PF20518
Domain description: Anaphase-promoting complex subunit 1 middle domain
Occurring in:
  1. Anaphase-promoting complex subunit 1
The deposited structure of PDB entry 6tlj contains 1 copy of Pfam domain PF20518 (Anaphase-promoting complex subunit 1 middle domain) in Anaphase-promoting complex subunit 1. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR001680
IPR018860
Domain description: Anaphase-promoting complex, subunit CDC26
Occurring in:
  1. Anaphase-promoting complex subunit CDC26
IPR013212
Domain description: Mad3/Bub1 homology region 1
Occurring in:
  1. Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
IPR004939
Domain description: APC10/DOC domain
Occurring in:
  1. Anaphase-promoting complex subunit 10
IPR024977
Domain description: Anaphase-promoting complex subunit 4-like, WD40 domain
Occurring in:
  1. Anaphase-promoting complex subunit 4
  2. Cell division cycle protein 20 homolog
  3. Cell division cycle protein 20 homolog
IPR036322
Domain description: WD40-repeat-containing domain superfamily
Occurring in:
  1. Anaphase-promoting complex subunit 4
  2. Cell division cycle protein 20 homolog
  3. Cell division cycle protein 20 homolog
IPR015943
Domain description: WD40/YVTN repeat-like-containing domain superfamily
Occurring in:
  1. Anaphase-promoting complex subunit 4
  2. Cell division cycle protein 20 homolog
  3. Cell division cycle protein 20 homolog
IPR003511
Domain description: HORMA domain
Occurring in:
  1. Mitotic spindle assembly checkpoint protein MAD2A
IPR036570
Domain description: HORMA domain superfamily
Occurring in:
  1. Mitotic spindle assembly checkpoint protein MAD2A
IPR033010
Domain description: WD repeat Cdc20/Fizzy
Occurring in:
  1. Cell division cycle protein 20 homolog
  2. Cell division cycle protein 20 homolog
IPR026000
Domain description: Anaphase-promoting complex subunit 5 domain
Occurring in:
  1. Anaphase-promoting complex subunit 5
IPR016158
Domain description: Cullin homology domain
Occurring in:
  1. Anaphase-promoting complex subunit 2
IPR008401
Domain description: Apc13
Occurring in:
  1. Anaphase-promoting complex subunit 13
IPR016901
Domain description: Anaphase-promoting complex subunit APC10/Doc1
Occurring in:
  1. Anaphase-promoting complex subunit 10
IPR029641
Domain description: Anaphase-promoting complex subunit 16
Occurring in:
  1. Anaphase-promoting complex subunit 16
IPR026182
Domain description: Anaphase-promoting complex subunit 15
Occurring in:
  1. Anaphase-promoting complex subunit 15
IPR007192
Domain description: Cdc23
Occurring in:
  1. Cell division cycle protein 23 homolog
IPR024991
Domain description: Anaphase-promoting complex subunit 11, RING-H2 finger
Occurring in:
  1. Anaphase-promoting complex subunit 11
IPR011989
Domain description: Armadillo-like helical
Occurring in:
  1. Anaphase-promoting complex subunit 1
IPR024990
Domain description: Anaphase-promoting complex subunit 1
Occurring in:
  1. Anaphase-promoting complex subunit 1
IPR045091
Domain description: Mad2-like
Occurring in:
  1. Mitotic spindle assembly checkpoint protein MAD2A
IPR011009
Domain description: Protein kinase-like domain superfamily
Occurring in:
  1. Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
IPR015661
Domain description: Mitotic spindle checkpoint protein Bub1/Mad3
Occurring in:
  1. Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
IPR037679
Domain description: Anaphase-promoting complex subunit 5
Occurring in:
  1. Anaphase-promoting complex subunit 5
IPR014786
Domain description: Anaphase-promoting complex subunit 2, C-terminal
Occurring in:
  1. Anaphase-promoting complex subunit 2
IPR036390
Domain description: Winged helix DNA-binding domain superfamily
Occurring in:
  1. Anaphase-promoting complex subunit 2
IPR036388
Domain description: Winged helix-like DNA-binding domain superfamily
Occurring in:
  1. Anaphase-promoting complex subunit 2
IPR001373
Domain description: Cullin, N-terminal
Occurring in:
  1. Anaphase-promoting complex subunit 2
IPR036317
Domain description: Cullin homology domain superfamily
Occurring in:
  1. Anaphase-promoting complex subunit 2
IPR044554
Domain description: Anaphase-promoting complex subunit 2
Occurring in:
  1. Anaphase-promoting complex subunit 2
IPR008979
Domain description: Galactose-binding-like domain superfamily
Occurring in:
  1. Anaphase-promoting complex subunit 10
IPR024790
Domain description: Anaphase-promoting complex subunit 4, long domain
Occurring in:
  1. Anaphase-promoting complex subunit 4
IPR017169
Domain description: Anaphase-promoting complex subunit 4, metazoa
Occurring in:
  1. Anaphase-promoting complex subunit 4
IPR024789
Domain description: Anaphase-promoting complex subunit 4
Occurring in:
  1. Anaphase-promoting complex subunit 4
IPR001841
Domain description: Zinc finger, RING-type
Occurring in:
  1. Anaphase-promoting complex subunit 11
IPR013083
Domain description: Zinc finger, RING/FYVE/PHD-type
Occurring in:
  1. Anaphase-promoting complex subunit 11
IPR041221
Domain description: Anaphase-promoting complex subunit 1, C-terminal
Occurring in:
  1. Anaphase-promoting complex subunit 1
IPR046794
Domain description: Anaphase-promoting complex subunit 1, middle domain
Occurring in:
  1. Anaphase-promoting complex subunit 1