› 6tnf

Electron Microscopy
3.8Å resolution

Structure of monoubiquitinated FANCD2 in complex with FANCI and DNA

Released:
DOI: 10.2210/pdb6tnf/pdb
PDB entry 6tnf coloured by chain, front view.
PDB entry 6tnf coloured by chain, side view.
PDB entry 6tnf coloured by chain, top view.
Source organisms:
Primary publication:
FANCD2-FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair.
Alcón P, Shakeel S, Chen ZA, Rappsilber J, Patel KJ, Passmore LA
OpenAccess logo Nat Struct Mol Biol 27 240-248 (2020)
PMID: 32066963
Related structures: EMD-10531

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-281235 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (4 distinct):
Fanconi anemia protein FANCD2 Chain: A
Molecule details ›
Chain: A
Length: 1439 amino acids
Theoretical weight: 160.93 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q68Y81 (Residues: 1-1438; Coverage: 100%)
Gene name: fancd2
Sequence domains: Fanconi anaemia protein FancD2 nuclease
Fanconi anemia complementation group I Chain: B
Molecule details ›
Chain: B
Length: 1338 amino acids
Theoretical weight: 149.46 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: B0I564 (Residues: 1-1338; Coverage: 100%)
Gene name: FANCI
Sequence domains:
Ubiquitin Chain: C
DNA (33-MER) Chains: D, E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-10531
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

19 review citations

The role of ALDH2 in tumorigenesis and tumor progression: Targeting ALDH2 as a potential cancer treatment.
Zhang et al. (2021)
18 more

3 mentions without citation

Structural insight into FANCI-FANCD2 monoubiquitination.
Li et al. (2020)
2 more