› 6to4

X-ray diffraction
2.29Å resolution

Imine Reductase from Myxococcus stipitatus in complex with NADP+

Released:
DOI: 10.2210/pdb6to4/pdb
PDB entry 6to4 coloured by chain, front view.
PDB entry 6to4 coloured by chain, side view.
PDB entry 6to4 coloured by chain, top view.
Source organism: Myxococcus stipitatus DSM 14675
Primary publication:
Inverting the Stereoselectivity of an NADH-Dependent Imine-Reductase Variant
Stockinger P, Borlinghaus N, Sharma M, Aberle B, Grogan G, Pleiss J, Nestl B
ChemCatChem (2021)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coenzyme F420-dependent NADP oxidoreductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 291 amino acids
Theoretical weight: 31.53 KDa
Source organism: Myxococcus stipitatus DSM 14675
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L7U9F5 (Residues: 1-291; Coverage: 100%)
Gene name: MYSTI_01767
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NAP 3 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 118.707Å b: 42.584Å c: 121.404Å
α: 90° β: 110.37° γ: 90°
R-values:
R R work R free
0.213 0.212 0.24
Expression system: Escherichia coli BL21(DE3)

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