› 6ttr

X-ray diffraction
2.85Å resolution

Crystal Structure of the coiled coil and GGDEF domain of DgcB from Caulobacter crescentus in complex with c-di-GMP

Released:
DOI: 10.2210/pdb6ttr/pdb
PDB entry 6ttr coloured by chain, front view.
PDB entry 6ttr coloured by chain, side view.
PDB entry 6ttr coloured by chain, top view.
Source organism: Caulobacter vibrioides NA1000
Entry authors: Holzschuh F, Schirmer T, Teixeira RD

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 24 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3CAN8 (Residues: 153-353; Coverage: 57%)
Gene names: CCNA_01926, dgcB
Sequence domains: Diguanylate cyclase, GGDEF domain

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand C2E 3 x C2E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 88.084Å b: 148.745Å c: 60.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.266
Expression system: Escherichia coli BL21(DE3)

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