› 6tug

X-ray diffraction
2.42Å resolution

Enterococcus italicus Csm6 bound to cyclic hexa-2'-fluoro-hexa-dAMP

Released:
DOI: 10.2210/pdb6tug/pdb
PDB entry 6tug coloured by chain, front view.
PDB entry 6tug coloured by chain, side view.
PDB entry 6tug coloured by chain, top view.
Source organism: Enterococcus italicus DSM 15952
Primary publication:
Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6.
Garcia-Doval C, Schwede F, Berk C, Rostøl JT, Niewoehner O, Tejero O, Hall J, Marraffini LA, Jinek M
OpenAccess logo Nat Commun 11 1596 (2020)
PMID: 32221291

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR system endoribonuclease Csm6 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 433 amino acids
Theoretical weight: 50.97 KDa
Source organism: Enterococcus italicus DSM 15952
Expression system: Escherichia coli
UniProt:
  • Canonical: E6LHV2 (Residues: 1-430; Coverage: 100%)
Gene names: HMPREF9088_1942, csm6
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand AF2 24 x AF2
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 93.604Å b: 106.686Å c: 116.793Å
α: 90.033° β: 90.007° γ: 90.1°
R-values:
R R work R free
0.265 0.264 0.292
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Controlling and enhancing CRISPR systems.
Shivram et al. (2021)
14 more