› 6tuo

X-ray diffraction
1.8Å resolution

Crystal structure of Archaeoglobus fulgidus Argonaute protein with cognate DNA oligoduplex 5'-pATTGTACGTACAAT

Released:
DOI: 10.2210/pdb6tuo/pdb
PDB entry 6tuo coloured by chain, front view.
PDB entry 6tuo coloured by chain, side view.
PDB entry 6tuo coloured by chain, top view.
Source organisms:
Entry authors: Grazulis S, Zaremba M

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120656 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Piwi protein Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 50.92 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28951 (Residues: 1-427; Coverage: 100%)
Gene name: AF_1318
Sequence domains: Piwi domain
5'-D(*AP*TP*TP*GP*TP*AP*CP*GP*TP*AP*CP*AP*AP*TP)-3' Chains: R, S
Molecule details ›
Chains: R, S
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand BME 3 x BME
Ligand PEG 1 x PEG
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Unit cell:
a: 52.011Å b: 99.634Å c: 109.802Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.184 0.227
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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