› 6twi

X-ray diffraction
2.27Å resolution

Crystal structure of the haemagglutinin mutant (Gln226Leu, Gly228Ser) from an H10N7 seal influenza virus isolated in Germany in complex with avian receptor analogue 3'-SLN

Released:
DOI: 10.2210/pdb6twi/pdb
PDB entry 6twi coloured by chain, front view.
PDB entry 6twi coloured by chain, side view.
PDB entry 6twi coloured by chain, top view.
Source organism: Influenza A virus (A/harbour seal/Germany/1/2014(H10N7))
Primary publication:
Hemagglutinin Traits Determine Transmission of Avian A/H10N7 Influenza Virus between Mammals.
Herfst S, Zhang J, Richard M, McBride R, Lexmond P, Bestebroer TM, Spronken MIJ, de Meulder D, van den Brand JM, Rosu ME, Martin SR, Gamblin SJ, Xiong X, Peng W, Bodewes R, van der Vries E, Osterhaus ADME, Paulson JC, Skehel JJ, Fouchier RAM
Cell Host Microbe 28 602-613.e7 (2020)
PMID: 33031770

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100836 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 325 amino acids
Theoretical weight: 35.51 KDa
Source organism: Influenza A virus (A/harbour seal/Germany/1/2014(H10N7))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A0A7HR51 (Residues: 10-332; Coverage: 59%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 177 amino acids
Theoretical weight: 20.31 KDa
Source organism: Influenza A virus (A/harbour seal/Germany/1/2014(H10N7))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A0A7HR51 (Residues: 333-508; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 5 x NAG
Ligand EDO 4 x EDO
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 64.843Å b: 215.101Å c: 78.246Å
α: 90° β: 96.78° γ: 90°
R-values:
R R work R free
0.247 0.245 0.273
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

Hemagglutinin Stability and Its Impact on Influenza A Virus Infectivity, Pathogenicity, and Transmissibility in Avians, Mice, Swine, Seals, Ferrets, and Humans.
Russell CJ. (2021)
4 more

1 mention without citation

Hemagglutinin Traits Determine Transmission of Avian A/H10N7 Influenza Virus between Mammals.
Herfst et al. (2020)