› 6tza

Electron Microscopy
7.2Å resolution

CryoEM reconstruction of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant

Released:
DOI: 10.2210/pdb6tza/pdb
PDB entry 6tza coloured by chain, front view.
PDB entry 6tza coloured by chain, side view.
PDB entry 6tza coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Membrane constriction and thinning by sequential ESCRT-III polymerization.
Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR, Iwasa JH, Vershinin MD, Sundquist WI, Frost A
OpenAccess logo Nat Struct Mol Biol 27 392-399 (2020)
PMID: 32251413
Related structures: EMD-20591

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IST1 homolog Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 189 amino acids
Theoretical weight: 21.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53990 (Residues: 1-189; Coverage: 52%)
  • Best match: P53990-6 (Residues: 1-41)
Gene names: IST1, KIAA0174
Sequence domains: Regulator of Vps4 activity in the MVB pathway

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.2Å
Relevant EMDB volumes: EMD-20591
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The ESCRTs - converging on mechanism.
Remec Pavlin et al. (2020)
5 more

1 mention without citation

Membrane constriction and thinning by sequential ESCRT-III polymerization.
Nguyen et al. (2020)

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 6tza found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-10398
Datasets: 1
Total size: 1.7Gb
EMPIAR thumbnail