› 6u01

X-ray diffraction
1.87Å resolution

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, N84D mutant with pyruvate bound in the active site

Released:
DOI: 10.2210/pdb6u01/pdb
PDB entry 6u01 coloured by chain, front view.
PDB entry 6u01 coloured by chain, side view.
PDB entry 6u01 coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase.
Majdi Yazdi M, Saran S, Mrozowich T, Lehnert C, Patel TR, Sanders DAR, Palmer DRJ
J Struct Biol 209 107409 (2020)
PMID: 31678256

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-576654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 310 amino acids
Theoretical weight: 34.19 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PPB4 (Residues: 1-298; Coverage: 100%)
Gene names: Cj0806, dapA
Sequence domains: Dihydrodipicolinate synthetase family

Ligands and Environments

7 bound ligands:
Ligand EDO 11 x EDO
Ligand PG4 1 x PG4
Ligand MG 6 x MG
Ligand PGE 4 x PGE
Ligand ACT 6 x ACT
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
1 modified residue:
Ligand KPI 6 x KPI

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: C2221
Unit cell:
a: 84.99Å b: 231.42Å c: 200.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.221
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Nanoscale Structure Determination of Murray Valley Encephalitis and Powassan Virus Non-Coding RNAs.
Mrozowich et al. (2020)
1 more