› 6u0x

X-ray diffraction
1.86Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS Q123D at cryogenic temperature

Released:
DOI: 10.2210/pdb6u0x/pdb
PDB entry 6u0x coloured by chain, front view.
PDB entry 6u0x coloured by chain, side view.
PDB entry 6u0x coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Toward the computational design of protein crystals with improved resolution.
Jeliazkov JR, Robinson AC, García-Moreno E B, Berger JM, Gray JJ
Acta Crystallogr D Struct Biol 75 1015-1027 (2019)
PMID: 31692475

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.13 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 70%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand THP 1 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 30.853Å b: 60.715Å c: 38.119Å
α: 90° β: 92.65° γ: 90°
R-values:
R R work R free
0.211 0.208 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Redefining Protein Interfaces within Protein Single Crystals with DNA.
Partridge et al. (2021)
1 more

1 mention without citation

Toward the computational design of protein crystals with improved resolution.
Jeliazkov et al. (2019)