› 6u13

X-ray diffraction
1.52Å resolution

Crystal Structure of the metallo-beta-lactamase L1 from Stenotrophomonas maltophilia in the complex with the hydrolyzed antibiotic moxalactam

Released:
DOI: 10.2210/pdb6u13/pdb
PDB entry 6u13 coloured by chain, front view.
PDB entry 6u13 coloured by chain, side view.
PDB entry 6u13 coloured by chain, top view.
Source organism: Stenotrophomonas maltophilia K279a
Entry authors: Kim Y, Maltseva N, Endres M, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 29.17 KDa
Source organism: Stenotrophomonas maltophilia K279a
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2FTM1 (Residues: 19-290; Coverage: 100%)
Gene name: Smlt2667
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand MX1 1 x MX1
Ligand ZN 2 x ZN
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6422
Unit cell:
a: 103.918Å b: 103.918Å c: 98.929Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.129 0.128 0.16
Expression system: Escherichia coli BL21(DE3)

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