› 6u3d

X-ray diffraction
1.75Å resolution

1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex

Released:
DOI: 10.2210/pdb6u3d/pdb
PDB entry 6u3d coloured by chain, front view.
PDB entry 6u3d coloured by chain, side view.
PDB entry 6u3d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Arrhythmia mutations in calmodulin can disrupt cooperativity of Ca2+ binding and cause misfolding.
Wang K, Brohus M, Holt C, Overgaard MT, Wimmer R, Van Petegem F
J Physiol 598 1169-1186 (2020)
PMID: 32012279

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143947 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Voltage-dependent L-type calcium channel subunit alpha-1C Chains: C, D
Molecule details ›
Chains: C, D
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13936 (Residues: 1659-1692; Coverage: 2%)
Gene names: CACH2, CACN2, CACNA1C, CACNL1A1, CCHL1A1

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 59.4Å b: 43.369Å c: 66.962Å
α: 90° β: 111.93° γ: 90°
R-values:
R R work R free
0.165 0.164 0.201
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Calmodulin Mutations in Human Disease.
Hussey et al. (2023)
1 more