› 6u44

X-ray diffraction
2.1Å resolution

Crystal structure of Methanoperedens nitroreducens elongation factor 2 H595N bound to GMPPCP and magnesium (monoclinic crystal form)

Released:
DOI: 10.2210/pdb6u44/pdb
PDB entry 6u44 coloured by chain, front view.
PDB entry 6u44 coloured by chain, side view.
PDB entry 6u44 coloured by chain, top view.
Source organism: Candidatus Methanoperedens nitroreducens
Primary publication:
Structural basis of elongation factor 2 switching
Fenwick MK, Ealick SE
Curr Res Struct Biol 2 25-34 (2020)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elongation factor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 753 amino acids
Theoretical weight: 83.3 KDa
Source organism: Candidatus Methanoperedens nitroreducens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A062V290 (Residues: 1-729; Coverage: 100%)
Gene names: ANME2D_00299, fusA
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand GCP 2 x GCP
Ligand MG 2 x MG
Ligand CL 4 x CL
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 78.322Å b: 106.814Å c: 98.056Å
α: 90° β: 105.46° γ: 90°
R-values:
R R work R free
0.189 0.187 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links