› 6u4v

X-ray diffraction
2.3Å resolution

Non-crosslinked wild type cysteine dioxygenase

Released:
DOI: 10.2210/pdb6u4v/pdb
PDB entry 6u4v coloured by chain, front view.
PDB entry 6u4v coloured by chain, side view.
PDB entry 6u4v coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The 3-His Metal Coordination Site Promotes the Coupling of Oxygen Activation to Cysteine Oxidation in Cysteine Dioxygenase.
Forbes DL, Meneely KM, Chilton AS, Lamb AL, Ellis HR
Biochemistry 59 2022-2031 (2020)
PMID: 32368901

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.04 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P43212
Unit cell:
a: 57.595Å b: 57.595Å c: 122.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.178 0.232
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Charge Maintenance during Catalysis in Nonheme Iron Oxygenases.
Traore et al. (2022)
1 more

2 mentions without citation

Crystal structure of human cysteamine dioxygenase provides a structural rationale for its function as an oxygen sensor.
Wang et al. (2021)
1 more