6u7g

X-ray diffraction
2.35Å resolution

HCoV-229E RBD Class V in complex with human APN

Released:
Model geometry
Data not analysed
Fit model/data
Data not analysed

Function and Biology Details

Reaction catalysed:
Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-147233 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Aminopeptidase N Chains: A, B
Molecule details ›
Chains: A, B
Length: 906 amino acids
Theoretical weight: 103.52 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P15144 (Residues: 66-967; Coverage: 93%)
Gene names: ANPEP, APN, CD13, PEPN
Sequence domains:
Spike glycoprotein Chains: C, D
Molecule details ›
Chains: C, D
Length: 145 amino acids
Theoretical weight: 15.85 KDa
Source organism: Human coronavirus 229E
Expression system: Homo sapiens
UniProt:
  • Canonical: Q1HVK2 (Residues: 292-432; Coverage: 24%)
Gene name: S
Sequence domains: Coronavirus spike glycoprotein S1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 99.555Å b: 98.682Å c: 147.525Å
α: 90° β: 104.6° γ: 90°
R-values:
R R work R free
0.181 0.179 0.218
Expression system: Homo sapiens