› 6u7y

X-ray diffraction
2.95Å resolution

RNA hairpin structure containing one TNA nucleotide as template

Released:
DOI: 10.2210/pdb6u7y/pdb
PDB entry 6u7y coloured by chain, front view.
PDB entry 6u7y coloured by chain, side view.
PDB entry 6u7y coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structural interpretation of the effects of threo-nucleotides on nonenzymatic template-directed polymerization.
Zhang W, Kim SC, Tam CP, Lelyveld VS, Bala S, Chaput JC, Szostak JW
Nucleic Acids Res 49 646-656 (2021)
PMID: 33347562

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197130 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (5'-R(*CP*UP*GP*CP*UP*GP*GP*CP*UP*AP*AP*GP*GP*(TG)P*CP*CP*GP*AP*AP*AP*GP*G)-3') Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 7.12 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*CP*UP*AP*UP*GP*CP*CP*UP*GP*CP*UP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.77 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand C5P 1 x C5P
1 modified residue:
Ligand TG 1 x TG

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: R3
Unit cell:
a: 68.766Å b: 68.766Å c: 69.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.182 0.256
Expression system: Not provided

Quick links

Citations

2 review citations

Prebiotic chemistry: a review of nucleoside phosphorylation and polymerization.
Guo et al. (2023)
1 more

1 mention without citation

Structural interpretation of the effects of threo-nucleotides on nonenzymatic template-directed polymerization.
Zhang et al. (2021)