› 6u81

X-ray diffraction
2.34Å resolution

Crystal Structure of the Double Homeodomain of DUX4 in Complex with a DNA aptamer

Released:
DOI: 10.2210/pdb6u81/pdb
PDB entry 6u81 coloured by chain, front view.
PDB entry 6u81 coloured by chain, side view.
PDB entry 6u81 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA aptamers against the DUX4 protein reveal novel therapeutic implications for FSHD.
Klingler C, Ashley J, Shi K, Stiefvater A, Kyba M, Sinnreich M, Aihara H, Kinter J
OpenAccess logo FASEB J 34 4573-4590 (2020)
PMID: 32020675

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120676 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Double homeobox protein 4 Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9UBX2 (Residues: 19-150; Coverage: 31%)
Gene names: DUX10, DUX4
Sequence domains: Homeodomain
DNA (5'-D(*GP*CP*GP*TP*AP*AP*TP*CP*TP*AP*AP*TP*CP*AP*AP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.18 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*GP*TP*TP*GP*AP*TP*TP*AP*GP*CP*CP*CP*AP*TP*TP*AP*CP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.1 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2221
Unit cell:
a: 71.64Å b: 73.19Å c: 108.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.247
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

10 review citations

Facioscapulohumeral muscular dystrophy: genetics, gene activation and downstream signalling with regard to recent therapeutic approaches: an update.
Schätzl et al. (2021)
9 more