› 6u8y

Electron Microscopy
4Å resolution

Structure of the membrane-bound sulfane sulfur reductase (MBS), an archaeal respiratory membrane complex

Released:
DOI: 10.2210/pdb6u8y/pdb
PDB entry 6u8y coloured by chain, front view.
PDB entry 6u8y coloured by chain, side view.
PDB entry 6u8y coloured by chain, top view.
Source organism: Pyrococcus furiosus COM1
Primary publication:
Structure of the respiratory MBS complex reveals iron-sulfur cluster catalyzed sulfane sulfur reduction in ancient life.
Yu H, Haja DK, Schut GJ, Wu CH, Meng X, Zhao G, Li H, Adams MWW
OpenAccess logo Nat Commun 11 5953 (2020)
PMID: 33230146
Related structures: EMD-20692

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 26-mer (preferred)
PDBe Complex ID:
PDB-CPX-125157 (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
Monovalent cation/H+ antiporter subunit E Chains: A, a
Molecule details ›
Chains: A, a
Length: 168 amino acids
Theoretical weight: 19.13 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6UQN5 (Residues: 1-168; Coverage: 100%)
Gene name: PFC_06500
Sequence domains: Na+/H+ ion antiporter subunit
Monovalent cation/H+ antiporter subunit F Chains: B, b
Molecule details ›
Chains: B, b
Length: 84 amino acids
Theoretical weight: 9.33 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6UZW2 (Residues: 1-84; Coverage: 100%)
Gene name: PFC_06495
Sequence domains: Multiple resistance and pH regulation protein F (MrpF / PhaF)
Monovalent cation/H+ antiporter subunit G Chains: C, c
Molecule details ›
Chains: C, c
Length: 124 amino acids
Theoretical weight: 13.55 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6TXQ5 (Residues: 1-124; Coverage: 100%)
Gene name: PFC_06490
Sequence domains: Na+/H+ antiporter subunit
MrpA C-terminal/MbhD domain-containing protein Chains: D, d
Molecule details ›
Chains: D, d
Length: 94 amino acids
Theoretical weight: 10.56 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6V2A4 (Residues: 1-94; Coverage: 100%)
Gene name: PFC_06485
Sequence domains: MBH, subunit D
Monovalent cation/H+ antiporter subunit B Chains: E, e
Molecule details ›
Chains: E, e
Length: 235 amino acids
Theoretical weight: 25.69 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6U860 (Residues: 1-235; Coverage: 100%)
Gene name: PFC_06480
Sequence domains:
Monovalent cation/H+ antiporter subunit C Chains: G, g
Molecule details ›
Chains: G, g
Length: 114 amino acids
Theoretical weight: 12.07 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6UQN0 (Residues: 1-114; Coverage: 100%)
Gene name: PFC_06475
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit domain-containing protein Chains: H, h
Molecule details ›
Chains: H, h
Length: 493 amino acids
Theoretical weight: 53.02 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6UZV7 (Residues: 1-493; Coverage: 100%)
Gene name: PFC_06470
Sequence domains: Proton-conducting membrane transporter
NADH:quinone oxidoreductase/Mrp antiporter membrane subunit domain-containing protein Chains: X, x
Molecule details ›
Chains: X, x
Length: 618 amino acids
Theoretical weight: 67.56 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6TXQ1 (Residues: 1-618; Coverage: 100%)
Gene name: PFC_06465
Sequence domains: Proton-conducting membrane transporter
NADH:ubiquinone oxidoreductase-like 20kDa subunit domain-containing protein Chains: J, j
Molecule details ›
Chains: J, j
Length: 192 amino acids
Theoretical weight: 22.73 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6UZV3 (Residues: 1-192; Coverage: 100%)
Gene name: PFC_06445
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
NADH:ubiquinone oxidoreductase 30kDa subunit domain-containing protein Chains: K, k
Molecule details ›
Chains: K, k
Length: 174 amino acids
Theoretical weight: 20.97 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6TXP7 (Residues: 1-174; Coverage: 100%)
Gene name: PFC_06440
Sequence domains: Respiratory-chain NADH dehydrogenase, 30 Kd subunit
NADH-quinone oxidoreductase subunit D domain-containing protein Chains: L, l
Molecule details ›
Chains: L, l
Length: 391 amino acids
Theoretical weight: 44.76 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6V297 (Residues: 1-391; Coverage: 100%)
Gene name: PFC_06435
Sequence domains: Respiratory-chain NADH dehydrogenase, 49 Kd subunit
NADH dehydrogenase subunit Chains: M, m
Molecule details ›
Chains: M, m
Length: 309 amino acids
Theoretical weight: 33.68 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6V2A1 (Residues: 1-309; Coverage: 100%)
Gene name: PFC_06460
Sequence domains: NADH dehydrogenase
4Fe-4S ferredoxin-type domain-containing protein Chains: N, n
Molecule details ›
Chains: N, n
Length: 208 amino acids
Theoretical weight: 24.75 KDa
Source organism: Pyrococcus furiosus COM1
UniProt:
  • Canonical: I6U853 (Residues: 1-208; Coverage: 100%)
Gene name: PFC_06430
Sequence domains: 4Fe-4S dicluster domain

Ligands and Environments

1 bound ligand:
Ligand SF4 6 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-20692

Quick links

Citations

1 review citation

Structure of respiratory complex I - An emerging blueprint for the mechanism.
Kampjut et al. (2022)
14 other articles

1 mention without citation

Evolution of complex I-like respiratory complexes.
Yu et al. (2021)