› 6uad

X-ray diffraction
1.75Å resolution

Ketosteroid isomerase (C. testosteroni) with truncated & designed loop for precise positioning of a catalytic E38

Released:
DOI: 10.2210/pdb6uad/pdb
PDB entry 6uad coloured by chain, front view.
PDB entry 6uad coloured by chain, side view.
PDB entry 6uad coloured by chain, top view.
Source organism: Comamonas testosteroni
Entry authors: Kundert K, Thompson MC, Liu L, Fraser JS, Kortemme T

Function and Biology Details

Reaction catalysed: 
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133705 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.44 KDa
Source organism: Comamonas testosteroni
Expression system: Escherichia coli
UniProt:
  • Canonical: P00947 (Residues: 1-125; Coverage: 99%)
Gene name: ksi
Sequence domains: SnoaL-like domain

Ligands and Environments

2 bound ligands:
Ligand DXC 2 x DXC
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6522
Unit cell:
a: 53.15Å b: 53.15Å c: 178.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.174 0.21
Expression system: Escherichia coli

Quick links