› 6uap

X-ray diffraction
2.74Å resolution

Crystal structure of tryptophan synthase from M. tuberculosis - open form with BRD6309 bound

Released:
DOI: 10.2210/pdb6uap/pdb
PDB entry 6uap coloured by chain, front view.
PDB entry 6uap coloured by chain, side view.
PDB entry 6uap coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Chang C, Michalska K, Maltseva NI, Jedrzejczak R, McCarren P, Nag PP, Joachimiak A, Satchell K, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161442 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tryptophan synthase alpha chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 276 amino acids
Theoretical weight: 28.58 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFY1 (Residues: 1-270; Coverage: 100%)
Gene names: MTCY01B2.05, Rv1613, trpA
Sequence domains: Tryptophan synthase alpha chain
Tryptophan synthase beta chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 410 amino acids
Theoretical weight: 43.56 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFX9 (Residues: 12-421; Coverage: 97%)
Gene names: MTCY01B2.04, Rv1612, trpB
Sequence domains: Pyridoxal-phosphate dependent enzyme

Ligands and Environments

6 bound ligands:
Ligand FMT 23 x FMT
Ligand EDO 2 x EDO
Ligand H9V 4 x H9V
Ligand MLA 3 x MLA
Ligand MLT 5 x MLT
Ligand ACT 6 x ACT
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 134.777Å b: 157.877Å c: 166.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.211
Expression system: Escherichia coli BL21(DE3)

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