› 6ubc

X-ray diffraction
1.65Å resolution

Crystal structure of a GH128 (subgroup VII) oligosaccharide-binding protein from Cryptococcus neoformans (CnGH128_VII)

Released:
DOI: 10.2210/pdb6ubc/pdb
PDB entry 6ubc coloured by chain, front view.
PDB entry 6ubc coloured by chain, side view.
PDB entry 6ubc coloured by chain, top view.
Source organism: Cryptococcus neoformans
Primary publication:
Structural insights into β-1,3-glucan cleavage by a glycoside hydrolase family.
Santos CR, Costa PACR, Vieira PS, Gonzalez SET, Correa TLR, Lima EA, Mandelli F, Pirolla RAS, Domingues MN, Cabral L, Martins MP, Cordeiro RL, Junior AT, Souza BP, Prates ÉT, Gozzo FC, Persinoti GF, Skaf MS, Murakami MT
Nat Chem Biol 16 920-929 (2020)
PMID: 32451508

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Asl1-like glycosyl hydrolase catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 46.71 KDa
Source organism: Cryptococcus neoformans
Expression system: Escherichia coli
UniProt:
  • Canonical: J9VNP7 (Residues: 21-443; Coverage: 100%)
Gene name: CNAG_04963
Sequence domains: Glycosyl hydrolase catalytic core

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I23
Unit cell:
a: 118.41Å b: 118.41Å c: 118.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.208
Expression system: Escherichia coli

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Citations

5 review citations

Recent advances in enzymatic synthesis of β-glucan and cellulose.
Bulmer et al. (2021)
4 more