› 6ubu

X-ray diffraction
1.6Å resolution

1.60 A resolution structure of the guanine riboswitch bound to guanine

Released:
DOI: 10.2210/pdb6ubu/pdb
PDB entry 6ubu coloured by chain, front view.
PDB entry 6ubu coloured by chain, side view.
PDB entry 6ubu coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
High Affinity Binding of N2-Modified Guanine Derivatives Significantly Disrupts the Ligand Binding Pocket of the Guanine Riboswitch.
Matyjasik MM, Hall SD, Batey RT
OpenAccess logo Molecules 25 (2020)
PMID: 32414072

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197137 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Guanine riboswitch aptamer domain Chain: B
Molecule details ›
Chain: B
Length: 67 nucleotides
Theoretical weight: 21.51 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
Sequence domains: Purine riboswitch

Ligands and Environments

3 bound ligands:
Ligand NCO 7 x NCO
Ligand ACT 3 x ACT
Ligand GUN 1 x GUN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 132.175Å b: 35.26Å c: 42.276Å
α: 90° β: 90.86° γ: 90°
R-values:
R R work R free
0.202 0.2 0.22
Expression system: Not provided

Quick links

Citations

1 review citation

A Riboswitch-Driven Era of New Antibacterials.
Giarimoglou et al. (2022)
4 other articles

4 mentions without citation

Riboswitches as Drug Targets for Antibiotics.
Panchal et al. (2021)
3 more